PMID: 8450018Feb 12, 1993Paper

Rapid, sensitive high-performance liquid chromatographic method for the quantification of promethazine in human serum with electrochemical detection

Journal of Chromatography
A R Fox, D A McLoughlin

Abstract

A method of analysis has been developed to quantify promethazine in human serum with a sensitivity that was suitable for bioavailability studies following a 50.0-mg rectal dose. The limit of quantification from 1.0 ml of serum for promethazine using electrochemical detection was 0.200 ng/ml. At this concentration, the total coefficient of variation obtained from seven replicates over the course of three days of validation was 7.53%. The amount of serum required, the ease of sample preparation and the precision of the method at the limit of quantification demonstrated an improvement over previous assays. A validation study was completed that included an evaluation of recovery, ruggedness, linearity of response, accuracy, precision, sensitivity, stability and selectivity. The method was then used to determine promethazine serum levels in a 36-subject bioavailability study following a 50.0-mg suppository dose.

Citations

Feb 20, 2010·European Journal of Drug Metabolism and Pharmacokinetics·Ping LiuRui-Chen Guo
Apr 13, 2007·Analytical and Bioanalytical Chemistry·Frank T Peters
May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zuzana BosákováEva Tesarová
Apr 1, 2012·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Tawfik A SalehAtta E Ibrahim
Feb 12, 1999·Ergonomics·M M Ayoub, P G Dempsey
Sep 12, 2008·Drug Development and Industrial Pharmacy·Ola A SalehAmr M Badawy
Nov 17, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·Q Song, L Putcha

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