Rapid separation and identification of major constituents in Pseudolarix kaempferi by ultra-performance liquid chromatography coupled with electrospray and quadrupole time-of-flight tandem mass spectrometry

Rapid Communications in Mass Spectrometry : RCM
Xia YeHaoyu Ye

Abstract

A rapid and reliable method based on ultra-performance liquid chromatography (UPLC) coupled with photodiode-array detection (PDA) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) has been developed for separation and identification of major constituents in extracts of root bark of Pseudolarix kaempferi Gordon (PKG). Identification of the constituents was carried out by interpretation of their retention times, UV absorption spectra, MS and MS/MS spectra, as well as the data provided by authentic standards and literatures. A total of 20 components were separated in only 8.0 min on a small particle size C18 column (1.7 microm). These components included nine diterpene acids, seven glycosides and four triterpenoids, among which pseudolaric acid C-O-beta-D-glucopyranoside and pseudolaric acid C2-O-beta-D-glucopyranoside were separated and identified for the first time in this study. Furthermore, the fragmentation patterns of the three types of compounds were elucidated for the first time. This established UPLC-PDA/Q-TOF-MS/MS method is reliable and effective for the separation and identification of the 20 compounds and will be useful for quality control of the crude materials of Pseudo...Continue Reading

References

Jan 28, 1993·The New England Journal of Medicine·D M EisenbergT L Delbanco
May 29, 1999·Journal of Natural Products·X C LiA M Clark
Nov 27, 2001·Bioorganic & Medicinal Chemistry Letters·S P Yang, J M Yue
Jul 27, 2002·Journal of Natural Products·Sheng-Ping YangJian-Min Yue
Oct 7, 2003·Bioorganic & Medicinal Chemistry·Sheng-Ping YangJian-Min Yue
Jul 14, 2004·Natural Product Research·Sheng-Ping YangJian-Min Yue
Sep 24, 2004·Rapid Communications in Mass Spectrometry : RCM·Robert PlumbAndrew Wright
Mar 4, 2005·Archives of Pharmacal Research·Xian-Feng GongTakashi Ikejima
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Vincent K W WongBen C B Ko
Jan 27, 2006·World Journal of Gastroenterology : WJG·Ke-Shen LiBin-You Wang
Jul 1, 2006·Phytochemistry·Peng LiuDean Guo
Nov 23, 2007·Acta Pharmacologica Sinica·Jing-hua YuTakashi Ikejima
Jul 19, 2008·Journal of Pharmacological Sciences·Jinghua YuTakashi Ikejima
Nov 13, 2008·Journal of the American Chemical Society·Barry M TrostArndt Meyer
Feb 17, 2009·Journal of Asian Natural Products Research·Ai-Guo Meng, Ling-Ling Jiang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.