Rapid separation of bacteriorhodopsin using a laminar-flow extraction system in a microfluidic device.

Biomicrofluidics
Yun Suk HuhTae Jung Park

Abstract

A protein separation technology using the microfluidic device was developed for the more rapid and effective analysis of target protein. This microfluidic separation system was carried out using the aqueous two-phase system (ATPS) and the ionic liquid two-phase system (ILTPS) for purification method of the protein sample, and the three-flow desalting system was used for the removal of salts from the sucrose-rich sample. Partitioning of the protein sample was observed in ATPS or ILTPS with the various pHs. The microdialysis system was applied to remove small molecules, such as sucrose and salts in the microfluidic channel with the different flow rates of buffer phase. A complex purification method, which combines microdialysis and ATPS or ILTPS, was carried out for the effective purification of bacteriorhodopsin (BR) from the purple membrane of Halobacterium salinarium, which was then analyzed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and matrix-assisted laser desorptionionization time-of-flight. Furthermore, we were able to make a stable three-phase flow controlling the flow rate in the microfluidic channel. Our complex purification methods were successful in purifying and recovering the BR to its required value.

References

Apr 26, 1990·Biochimica Et Biophysica Acta·R R Birge
Nov 1, 1985·Biophysical Journal·R M GlaeserR Henderson
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D Oesterhelt, W Stoeckenius
Sep 29, 1971·Nature: New Biology·D Oesterhelt, W Stoeckenius
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·U Gündüz, K Korkmaz
Nov 10, 2001·Electrophoresis·T FootzC J Backhouse
Mar 31, 2004·Lab on a Chip·Yung-Chiang ChungChia-Yu Fan
Jun 15, 2004·Analytical Chemistry·Torsten VilknerAndreas Manz
Feb 1, 2005·Analytical Chemistry·Sergey S ShevkoplyasMark W Bitensky
Sep 10, 1971·Nature·R E Eckardt
Jun 29, 2006·Molecular & Cellular Proteomics : MCP·Birgit BisleDieter Oesterhelt
Sep 12, 2009·Protein Science : a Publication of the Protein Society·Kazumi ShimonoShigeyuki Yokoyama

❮ Previous
Next ❯

Citations

Apr 16, 2013·Protein Expression and Purification·Pei-Jing ShiuCheng-Kang Lee
Sep 8, 2011·Lab on a Chip·Steffen Hardt, Thomas Hahn
Nov 22, 2019·Chemical Society Reviews·Youchuang Chao, Ho Cheung Shum
Dec 17, 2020·Comprehensive Reviews in Food Science and Food Safety·Leonardo M de Souza MesquitaVeridiana V de Rosso

❮ Previous
Next ❯

Methods Mentioned

BETA
chip
electrophoresis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.