Rapid separation of very low concentrations of bacteria from blood

Journal of Microbiological Methods
Clara M BuchananWilliam G Pitt

Abstract

A rapid and accurate diagnosis of the species and antibiotic resistance of bacteria in septic blood is vital to increase survival rates of patients with bloodstream infections, particularly those with carbapenem-resistant enterobacteriaceae (CRE) infections. The extremely low levels in blood (1 to 100CFU/ml) make rapid diagnosis difficult. In this study, very low concentrations of bacteria (6 to 200CFU/ml) were separated from 7ml of whole blood using rapid sedimentation in a spinning hollow disk that separated plasma from red and white cells, leaving most of the bacteria suspended in the plasma. Following less than a minute of spinning, the disk was slowed, the plasma was recovered, and the bacteria were isolated by vacuum filtration. The filters were grown on nutrient plates to determine the number of bacteria recovered from the blood. Experiments were done without red blood cell (RBC) lysis and with RBC lysis in the recovered plasma. While there was scatter in the data from blood with low bacterial concentrations, the mean average recovery was 69%. The gender of the blood donor made no statistical difference in bacterial recovery. These results show that this rapid technique recovers a significant amount of bacteria from bloo...Continue Reading

Citations

Oct 6, 2019·Molecular & Cellular Proteomics : MCP·Florence Roux-DalvaiArnaud Droit
Jul 28, 2020·Biotechnology Progress·Clifton M Anderson, William G Pitt
Aug 20, 2019·Biotechnology Progress·William G PittClifton M Anderson
Dec 1, 2019·Molecular & Cellular Proteomics : MCP·Florence Roux-DalvaiArnaud Droit
Oct 5, 2018·Analytical Chemistry·Laura PasitkaCarl-Fredrik Mandenius

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