Rapid, sequence-specific detection of unpurified PCR amplicons via a reusable, electrochemical sensor

Proceedings of the National Academy of Sciences of the United States of America
Rebecca Y LaiAlan J Heeger

Abstract

We report an electrochemical method for the sequence-specific detection of unpurified amplification products of the gyrB gene of Salmonella typhimurium. Using an asymmetric PCR and the electrochemical E-DNA detection scheme, single-stranded amplicons were produced from as few as 90 gene copies and, without subsequent purification, rapidly identified. The detection is specific; the sensor does not respond when challenged with control oligonucleotides based on the gyrB genes of either Escherichia coli or various Shigella species. In contrast to existing sequence-specific optical- and capillary electrophoresis-based detection methods, the E-DNA sensor is fully electronic and requires neither cumbersome, expensive optics nor high voltage power supplies. Given these advantages, E-DNA sensors appear well suited for implementation in portable PCR microdevices directed at, for example, the rapid detection of pathogens.

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Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·Kim-Fatt LowYean-Yean Chan
Mar 6, 2010·Accounts of Chemical Research·Arica A Lubin, Kevin W Plaxco
Aug 11, 2012·Journal of the American Chemical Society·Alireza Abi, Elena E Ferapontova
Sep 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Nicholas M AdamsDavid W Wright
Oct 11, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Weiwei Yang, Rebecca Y Lai
May 10, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Francesco RicciJames J Sumner
Jan 10, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Elizabeth PavlovicH T Soh
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Aug 5, 2009·Chemical Communications : Chem Comm·Socrates Jose P CañeteRebecca Y Lai
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Feb 18, 2014·Analytical Chemistry·Meihua LinQing Huang

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