PMID: 108320Feb 1, 1979

Rapid serotyping of groups A, B, and C meningococci by rocket-line immunoelectrophoresis and co-agglutination

Journal of Clinical Pathology
D Danielsson, P Olcén

Abstract

Rocket-line immunoelectrophoresis (R-LIE) with antigen containing intermediate gel, and co-agglutination utilising protein A-containing staphylococci coated with specific antibodies, were adapted for serotyping the prototypes of group B meningococci. Both were found to have the same specificity as agar gel double diffusion (AGDD) but they were more sensitive and more rapid than AGDD. R-LIE required, like AGDD, the extraction of relatively large quantities of bacteria, while the co-agglutination method, performed as a slide agglutination tests with results within a few minutes and no need of special equipment, required only a small amount of heated whole meningococcal cells. Meningococcal strains of serogroups B, C, and A from patients with carriers were serotyped and the results with all three methods were in agreement.

References

Jul 19, 1975·Lancet·J S FarriesD M Jones
Aug 1, 1977·The Journal of Infectious Diseases·M P GlodeL A Hanson
Mar 1, 1975·The Journal of Infectious Diseases·R S MunfordG W Gorman
Aug 1, 1976·Journal of Clinical Pathology·D M Jones, B M Tobin
Dec 1, 1971·The Journal of Infectious Diseases·R GoldM S Artenstein

Citations

Oct 1, 1984·The Journal of Hygiene·R J FallonW Lore
Feb 4, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Muhamed-Kheir Taha, P Olcén
Jan 1, 1988·Scandinavian Journal of Gastroenterology. Supplement·D DanielssonT U Kosunen
Jan 1, 1987·Scandinavian Journal of Immunology·R R KaldanyM J Lefford

Related Concepts

Agglutination Tests
Carrier of Disorder
Immunodiffusion Measurement
Immunoelectrophoresis
Meningococcal Infections
Neisseria meningitidis
Serotyping

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