Rapid, simple, and clinically applicable high-performance liquid chromatography method for clinical determination of plasma colistin concentrations

Journal of Pharmaceutical Health Care and Sciences
Yuki HanaiTakashi Yoshio

Abstract

Since both the antibacterial effects and common adverse effects of colistin are concentration-dependent, determination of the most appropriate dosage regimen and administration method for colistin therapy is essential to ensure its efficacy and safety. We aimed to establish a rapid and simple high-performance liquid chromatography (HPLC)-based system for the clinical determination of colistin serum concentrations. Extraction using a solid-phase C18 cartridge, derivatisation with 9-fluorenylmethyl chloroformate, and elution with a short reversed-phase Cl8 column effectively separated colistin from an internal standard. The HPLC apparatus and conditions were as follows: analytical column, Hydrosphere C18; sample injection volume, 50 μL; column temperature, 40 °C; detector, Shimadzu RF-5300 fluorescence spectrophotometer (excitation wavelength, 260 nm; emission wavelength, 315 nm); mobile phase, acetonitrile/tetrahydrofuran/distilled water (50,14,20, v/v/v); flow-rate, 1.6 mL/min. The calibration curves obtained for colistin were linear in the concentration range of 0.10-8.0 μg/mL. The regression equation was y = 0.6496× - 0.0141 (r2 = 0.9999). The limit of detection was ~ 0.025 μg/mL, and the assay intra- and inter-day precisions...Continue Reading

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Citations

May 21, 2021·Scientific Reports·Emilie ChironElaine L Ferguson

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Methods Mentioned

BETA
electrophoresis

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