Rapid, simple and efficient method for detection of viral genomes on raspberries

Journal of Virological Methods
A PerrinC Gantzer

Abstract

In recent years, foodborne viruses, especially human noroviruses (NoV) and hepatitis A virus (HAV), have been increasingly reported as the causes of foodborne disease outbreaks. Soft red fruits, especially raspberries, have a high incidence among the types of food concerned. Due to low infectious doses and low concentrations of enteric viruses in food samples, it is necessary to have an efficient and rapid detection method to implement prevention measures. A standard method for virus detection and quantification in food, including raspberries (XP CEN ISO/TS 15216-1 and -2, 2013) is currently available. This method proposes a consensus detection approach by RT-real time PCR (RT-qPCR) but also a virus extraction procedure based on the elution-concentration principle. In this study, an alternative method of extraction in which RNAs are directly extracted from food matrices (based on direct RNA extraction) has been optimized. First, each step was improved to make it a highly rapid, specific and simple method. Second, the standard virus concentration method was compared with the optimized direct RNA extraction one. Human enteric viral surrogates, Murine Norovirus (MNV) and F-specific RNA bacteriophage GA, were selected according to ...Continue Reading

References

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Citations

Nov 23, 2017·Frontiers in Microbiology·Anna KłopotKrystyna Dąbrowska
Jul 5, 2017·Food and Environmental Virology·Stéphanie Watier-GrillotV Pommier de Santi
Jul 12, 2020·Food and Environmental Virology·Sheikh Md RajiuddinAnna Charlotte Schultz
Oct 12, 2017·Food and Environmental Virology·Maija Summa, Leena Maunula
Apr 24, 2020·Food and Environmental Virology·Mitchie Y Zhao, Dan Li
Feb 11, 2021·Food and Environmental Virology·Sophie ButotSophie Zuber
Sep 19, 2017·Journal of Virological Methods·Yan Zheng, Yuan Hu

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