Rapid simultaneous diagnosis of infections with respiratory syncytial viruses A and B, influenza viruses A and B, and human parainfluenza virus types 1, 2, and 3 by multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (Hexaplex)

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
J FanL L Savatski

Abstract

A multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (Hexaplex; Prodesse, Milwaukee) was developed and used to rapidly detect and quantitate RNA of respiratory syncytial viruses A and B, influenza viruses A and B, and human parainfluenza virus types 1, 2, and 3 in nasal wash specimens in a single test. Primers and probes originated from highly conserved regions of each viral genome. Six and a half primer pairs were mixed for the simultaneous detection and quantitation of RNA from seven different respiratory viruses. We tested 109 clinical samples with this assay. Twenty-nine virus culture-positive samples were all positive by Hexaplex. Samples from 40 symptomatic patients were negative by virus culture, but eight of these were positive by Hexaplex. Forty samples from asymptomatic children were negative by both virus culture and Hexaplex. No cross-reactions were noted among 17 different respiratory viruses with use of this assay. Hexaplex was 100% sensitive (95% confidence interval [CI], 0.88-1.0) and 98% specific (95% CI, 0.97-0.99). All eight "false-positive" Hexaplex results (in comparison with negative viral culture results) were for symptomatic patients with low numbers of viru...Continue Reading

Citations

Nov 1, 2002·Reviews in Medical Virology·Joanna S Ellis, Maria C Zambon
Oct 1, 2008·Internal and Emergency Medicine·Carlos A CamargoDennis E Niewoehner
Nov 30, 2000·Current Infectious Disease Reports·T Heikkinen, T Chonmaitree
Mar 3, 2011·Current Infectious Disease Reports·Shu ZhangYi-Wei Tang
Apr 10, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Adriana WeinbergUNKNOWN ARIVAC consortium
Jan 9, 2003·Lancet·Terho Heikkinen, Asko Järvinen
Jun 9, 2001·Antiviral Research·A C SchmidtR M Chanock
Jul 10, 2002·Antiviral Research·Michael G IsonFrederick Hayden
Oct 9, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Adriana WeinbergTony N Hodges
Apr 1, 2010·Seminars in Respiratory and Critical Care Medicine·Pali Dedhiya Shah, John F McDyer
Jun 23, 2001·The New England Journal of Medicine·C B Hall
Jan 30, 2004·The New England Journal of Medicine·John V WilliamsJames E Crowe
Jun 19, 2012·Avian Pathology : Journal of the W.V.P.A·Erica Spackman
Feb 19, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Tasnee ChonmaitreeKelly J Henrickson
May 23, 2000·The Pediatric Infectious Disease Journal·T Heikkinen
May 1, 2002·Current Opinion in Pulmonary Medicine·Stephen B Greenberg
Jan 20, 2004·The Pediatric Infectious Disease Journal·Kelly J Henrickson
Nov 7, 2007·Transplantation·Francisco López-MedranoEnrique Moreno-González
Jan 3, 2008·The Pediatric Infectious Disease Journal·Kelly J Henrickson, Caroline Breese Hall
Nov 4, 2008·The Pediatric Infectious Disease Journal·Tuomas JarttiOlli Ruuskanen
Oct 28, 2009·The Pediatric Infectious Disease Journal·Janak A PatelTasnee Chonmaitree
Mar 24, 2010·The Pediatric Infectious Disease Journal·Stella U KaluTasnee Chonmaitree
Oct 16, 2008·Clinical Microbiology Reviews·James B Mahony
Apr 12, 2012·Clinical Microbiology Reviews·Swati Kumar, Kelly J Henrickson
Jan 11, 2000·Clinical Microbiology Reviews·W M Sullender
Oct 12, 2000·Clinical Microbiology Reviews·E M ElnifroP E Klapper
Apr 15, 2003·Clinical Microbiology Reviews·Kelly J Henrickson
Sep 7, 2007·Journal of Clinical Microbiology·W Y LamPaul K S Chan
Jun 29, 2007·Journal of Clinical Microbiology·Frederick S NolteJames R Prudent
Mar 13, 2009·Journal of Clinical Microbiology·Baochuan LinDavid A Stenger
Dec 17, 2008·Journal of Clinical Microbiology·Ying HuangElizabeth L Mather

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.