Rapid solution application methods

Current Protocols in Neuroscience
C M Tang

Abstract

Solution changes to deliver solutes of different compositions are required in virtually every cellular electrophysiological experiment. Also, in many neurobiology experiments, it is necessary to make rapid step changes in the concentration of a test compound in order to outpace receptor desensitization or to mimic the brief lifetime of a fast synaptic response. The goal of this unit is to aid the investigator in choosing the rapid solution application method that is most appropriate for the experimental situation and to describe the methods for fabricating two relatively simple devices for making rapid changes between different solutions.

References

May 23, 1985·Neuroscience Letters·W Boll, H D Lux
Sep 1, 1989·Pflügers Archiv : European journal of physiology·D J Maconochie, D E Knight
Nov 1, 1986·Biophysical Journal·R S BrettB Lancaster
Jan 1, 1980·Neuroscience·O A Krishtal, V I Pidoplichko
Mar 1, 1993·Pflügers Archiv : European journal of physiology·D S Koh, W Vogel

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Citations

Apr 2, 2015·The Journal of Biological Chemistry·Rita E SirriehVasanthi Jayaraman
Jul 15, 2015·The Journal of General Physiology·Rita E SirriehVasanthi Jayaraman

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