Rapid specific detection and quantification of bacteria and archaea involved in mineral sulfide bioleaching using real-time PCR

Biotechnology and Bioengineering
Chun-Qiang LiuPhilip Hendry

Abstract

A SybrGreen real-time PCR assay was developed to detect and quantify both total and selected 16S rDNA species of bacteria and archaea involved in the bioleaching of metals from sulfide ores. A set of specific and universal primers based on 16S rDNA sequences was designed and validated for specific detection and quantification of DNA isolated from representative strains of Acidianus brierleyi, Sulfolobus sp., Sulfobacillus thermosulfidooxidans, Sulfobacillus acidophilus, Acidithiobacillus caldus, and Leptospirillum ferrooxidans. An artificial sequence based on 16S rDNA was constructed to quantify total 16S rDNA in mixed DNA samples. The real-time PCR assay was further validated using a mixture of 16S rDNA amplicons derived from the six different species, each added at a known amount. Finally, the real-time PCR assay was used to monitor the change of 16S rDNA copies of four bioleaching strains inoculated into chalcopyrite airlift column reactors operated at different temperatures. The growth dynamics of these strains correlated well with the expected effects of temperature in the chalcopyrite-leaching environment. The suitability of this method for monitoring microbial populations in industrial bioleaching environments is discussed.

References

Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M BarnsN R Pace
Jul 1, 1997·Applied and Environmental Microbiology·P De Wulf-DurandL I Sly
May 2, 2000·Journal of Clinical Microbiology·C E CorlessA J Fox
May 11, 2000·Extremophiles : Life Under Extreme Conditions·P R NorrisN A Foulis
Nov 28, 2001·Applied and Environmental Microbiology·K TakaiJ K Fredrickson
Sep 1, 1994·Applied and Environmental Microbiology·A M AmaroC A Jerez
Jun 1, 1996·Applied and Environmental Microbiology·D L StonerM T Macdonell

❮ Previous
Next ❯

Citations

Jul 1, 2008·Applied and Environmental Microbiology·Dagmar Kock, Axel Schippers
Nov 19, 2013·Applied and Environmental Microbiology·Yuguang WangHongbo Zhou
Nov 3, 2006·Journal of Biotechnology·Tetsushi SuyamaTakahiro Kanagawa
May 6, 2016·Environmental Science and Pollution Research International·Shen-Yi Chen, Li-Chieh Chou
Jun 12, 2016·Research in Microbiology·Harold NuñezRaquel Quatrini
Apr 30, 2020·Zoonoses and Public Health·Amanda ShapiroJacqueline Norris
Apr 12, 2013·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Shen-Yi Chen, Wen-Hsing Chen
Jan 10, 2017·Frontiers in Microbiology·Sabrina HedrichCatherine Joulian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.