Rapid, stimulation-induced reduction of C12-resorufin in motor nerve terminals: linkage to mitochondrial metabolism

Journal of Neurochemistry
Janet D TalbotGavriel David

Abstract

The Alamar blue (resazurin) assay of cell viability monitors the irreversible reduction of non-fluorescent resazurin to fluorescent resorufin. This study focused on the reversible reduction of C12-resorufin to non-fluorescent C12-dihydroresorufin in motor nerve terminals innervating lizard intercostal muscles. Resting C12-resorufin fluorescence decreased when the activity of the mitochondrial electron transport chain (ETC) was accelerated with carbonyl cyanide m-chloro phenyl hydrazone, and increased when ETC activity was inhibited with cyanide. Trains of action potentials (50 Hz for 20-50 s), which reversibly decreased NADH fluorescence and partially depolarized the mitochondrial membrane potential, produced a reversible decrease in C12-resorufin fluorescence which had a similar time course. The stimulation-induced decrease in C12-resorufin fluorescence was blocked by inhibitors of ETC complexes I, III, and IV and by carbonyl cyanide m-chloro phenyl hydrazone, but not by inhibiting mitochondrial ATP synthesis with oligomycin. Mitochondrial depolarization and the decreases in C12-resorufin and NADH fluorescence depended on Ca2+ influx into the terminal, but not on vesicular transmitter release. These results suggest that the re...Continue Reading

References

Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J A ConnorC L Prosser
May 1, 1990·The American Journal of Physiology·T E Gunter, D R Pfeiffer
Jan 1, 1969·The Journal of Physiology·F A DodgeR Rahamimoff
Oct 22, 1982·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C B GundersenR Miledi
Mar 1, 1984·Archives of Biochemistry and Biophysics·R W NimsR A Lubet
Dec 28, 1996·Journal of Neuroscience Methods·M J WhiteD A Greenberg
Nov 14, 1997·The Journal of Physiology·G DavidE F Barrett
Apr 18, 1998·Journal of Neurophysiology·M WakamoriY Mori
Jun 19, 1998·Brain Research. Brain Research Protocols·J E SpringerS L Carlson
Nov 7, 1998·International Journal of Andrology·A A ZalataF H Comhaire
Jul 25, 2000·Brain Research. Brain Research Protocols·E Magnani, E Bettini
May 30, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R J Gonzalez, J B Tarloff
Feb 26, 2003·Cell Calcium·Gavriel DavidEllen F Barrett
Mar 28, 2003·The Journal of Biological Chemistry·Susan Chalmers, David G Nicholls
Aug 17, 1962·Science·B CHANCEB SCHOENER
Jan 23, 2004·Nature·Yuriy KirichokDavid E Clapham
Oct 4, 2005·Archives of Pathology & Laboratory Medicine·Khaled K Abu-Amero, Thomas M Bosley
Sep 1, 2006·American Journal of Physiology. Cell Physiology·Avraham Mayevsky, Gennady G Rogatsky
Jan 1, 1947·Annual Review of Biochemistry·L MICHAELIS

❮ Previous
Next ❯

Citations

Jan 20, 2012·The Journal of Antimicrobial Chemotherapy·Manal J NattoHarry P de Koning
Jan 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amit K ChouhanGregory T Macleod
Mar 31, 2010·Life Sciences·Yoko NishinakaToshiyuki Arai
Oct 13, 2015·Environmental Science & Technology·Jian Lin ChenDavid C Stuckey
Mar 17, 2010·Journal of the American Chemical Society·Oleksandr A KucherakAndrey S Klymchenko
Oct 28, 2008·Nitric Oxide : Biology and Chemistry·Cezar R PestanaCarlos Curti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.