PMID: 3766969Aug 15, 1986Paper

Rapid sulfopropyl-disk chromatographic purification of bovine and human thrombin

Analytical Biochemistry
F C Church, H C Whinna

Abstract

Thrombin, from either a crude commercial preparation (bovine) or a prothrombin activation mixture (human), was purified by sulfopropyl-disk chromatography to homogeneity in a rapid and convenient single-step procedure. The yield of both proteinases was greater than 85%. Purified bovine and human thrombin had sp act of 2500 and 3000 "NIH" units/mg, respectively. Human thrombin was more reactive than bovine thrombin with these active site-directed probes: phenylmethylsulfonyl fluoride, dansyl-Glu-Gly-Arg-chloromethylketone, and human heparin cofactor II. The sulfopropyl-disk chromatographic method is a useful and rapid technique to prepare milligram quantities of highly purified bovine and human thrombin.

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Citations

Dec 1, 1993·Journal of Protein Chemistry·H C Whinna, F C Church
Mar 1, 1996·Thrombosis Research·W T SumnerM Hoffman
Jan 5, 1995·Biochimica Et Biophysica Acta·S T Cooper, F C Church
Oct 1, 2003·Experimental and Molecular Pathology·Maureane HoffmanFrank C Church
Feb 1, 1995·Preparative Biochemistry·Z Ding, Y Xu
Jan 29, 2016·Thrombosis and Haemostasis·Romy M W KremersVera Ignjatovic
Feb 8, 2011·Journal of Thrombosis and Haemostasis : JTH·M HoffmanD M Monroe
Nov 26, 2014·Journal of Thrombosis and Haemostasis : JTH·R M W KremersH C Hemker
Mar 15, 1989·Thrombosis Research·C W PrattF C Church
Jan 1, 1989·Annals of the New York Academy of Sciences·C W PrattF C Church
Oct 14, 1987·Biochemical and Biophysical Research Communications·F C ChurchH C Whinna
Aug 5, 2004·The Journal of Biological Chemistry·Yolanda M FortenberryFrank C Church
Sep 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·R A ShirkW D Wagner
Jan 15, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·J A OliverM Hoffman
Nov 27, 1999·The Journal of Biological Chemistry·S J Bauman, F C Church
Aug 23, 2002·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·A S WolbergM Hoffman
Nov 9, 2014·The Journal of Biological Chemistry·Adam MisztaBas de Laat
Apr 23, 2008·Current Protocols in Protein Science·Susannah J BaumanFrank C Church
Jul 29, 2020·Scientific Reports·Romy M W de Laat-KremersBas de Laat
Jan 1, 1995·International Journal of Peptide and Protein Research·L G MeltonB W Erickson
Jul 2, 1998·Archives of Biochemistry and Biophysics·L L NeeseF C Church
Dec 15, 1994·Thrombosis Research·J ChabbatM Steinbuch
Aug 1, 1988·Analytical Biochemistry·D M MonroeH R Roberts
Oct 1, 1989·Revue française de transfusion et d'hémobiologie : bulletin de la Société nationale de transfusion sanguine·J L LorneE Boschetti
Mar 15, 1988·Biochemical and Biophysical Research Communications·V W ChanC L Borders

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