Rapid susceptibility profiling of carbapenem-resistant Klebsiella pneumoniae

Scientific Reports
K T MulroneyT J J Inglis

Abstract

The expanding global distribution of multi-resistant Klebsiella pneumoniae demands faster antimicrobial susceptibility testing (AST) to guide antibiotic treatment. Current ASTs rely on time-consuming differentiation of resistance and susceptibility after initial isolation of bacteria from a clinical specimen. Here we describe a flow cytometry workflow to determine carbapenem susceptibility from bacterial cell characteristics in an international K. pneumoniae isolate collection (n = 48), with a range of carbapenemases. Our flow cytometry-assisted susceptibility test (FAST) method combines rapid qualitative susceptible/non-susceptible classification and quantitative MIC measurement in a single process completed shortly after receipt of a primary isolate (54 and 158 minutes respectively). The qualitative FAST results and FAST-derived MIC (MICFAST) correspond closely with broth microdilution MIC (MICBMD, Matthew's correlation coefficient 0.887), align with the international AST standard (ISO 200776-1; 2006) and could be used for rapid determination of antimicrobial susceptibility in a wider range of Gram negative and Gram positive bacteria.

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Citations

May 31, 2019·Journal of Medical Microbiology·Timothy J J Inglis, Oskar Ekelund
Sep 7, 2018·Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis·Kieran T MulroneyAron Chakera
Aug 14, 2020·The Journal of Antimicrobial Chemotherapy·Anna ÅkerlundUNKNOWN RAST Study Group
Oct 23, 2020·Nature Communications·Daniel C SpencerHywel Morgan
Nov 27, 2018·Analytical Chemistry·Morteza AziziAlireza Abbaspourrad

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Methods Mentioned

BETA
PCR
flow cytometry
FCS
fluorescence microscopy

Software Mentioned

Flow
GraphPad
FlowJo
MIC FAST
FAST
FCS
Prism

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