Rapid susceptibility testing of fungi by flow cytometry using vital staining.

Journal of Clinical Microbiology
C WenischA Georgopoulos

Abstract

A 1-h assay for antifungal susceptibility testing measuring the impairment of fungal metabolic activity was developed. Yeast viability was analyzed by flow cytometry with a novel fluorescent probe, FUN-1, which emits a red fluorescence when the yeast is metabolically active. For nine Candida albicans strains tested, this method yielded results comparable to those obtained by the standard M27 procedure for amphotericin B, flucytosine, fluconazole, and ketoconazole. Whether the flow cytometry antifungal susceptibility test results correlate with the in vivo activities of the drugs remains to determined.

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Citations

Mar 29, 2000·Journal of Applied Microbiology·S D CoxS G Wyllie
May 1, 2020·Clinical Microbiology Reviews·Elizabeth L BerkowLuis Ostrosky-Zeichner
Oct 5, 2001·Clinical Microbiology Reviews·J H RexD W Warnock
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Jan 10, 2002·Infectious Disease Clinics of North America·M A Pfaller, W L Yu

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