Rapid synthesis of a register-specific heterotrimeric type I collagen helix encompassing the integrin alpha2beta1 binding site.

Journal of Molecular Biology
David A SlatterR W Farndale

Abstract

We report a rapid method to synthesize cystine cross-linked heterotrimeric collagenous peptides. They can be engineered to favour one particular axial alignment of the strands, called the register of the helix. Here, the sequence of the constituent peptides contains 18 residues of "guest" collagen type I sequence flanked by N and C-terminal (Gly-Pro-Pro)5 "host" modules which ensure helicity. Further C-terminal residues include appropriately spaced cysteine residues and alanine to provide the necessary flexibility for helix formation. The cross-linking reaction and subsequent separation protocols have been designed for any inserted collagen sequence that does not contain a cysteine residue. Mass spectrometry and ion-exchange chromatography allow us to distinguish between different disulphide-bonded species and to monitor the formation of side-products. Starting peptide can be recovered simply from the reaction mixture by reduction and separation. Yields are typically 30%, working on a 10 mg scale. 15N-1H NMR and platelet adhesion studies show that the peptide heterotrimers presented here can reshuffle to cover all three axial registers. Less flexible spacers between the disulphide linkages and the helix will restrict each heter...Continue Reading

References

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Citations

Sep 19, 2008·Journal of the American Chemical Society·Balaraman MadhanBarbara Brodsky
Jun 20, 2008·Nature·Barbara Brodsky, Jean Baum
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michèle C EratIoannis Vakonakis
Jul 25, 2009·The Journal of Biological Chemistry·Jorge A FallasJeffrey D Hartgerink
Oct 27, 2007·Antioxidants & Redox Signaling·Cyril BoulègueLuis Moroder
Mar 6, 2010·Organic & Biomolecular Chemistry·Gregg B Fields
Jan 29, 2016·The Journal of Physical Chemistry. B·Chu-Harn Chiang, Jia-Cherng Horng
Dec 5, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Rita BerisioLuigi Vitagliano
Sep 8, 2011·Angewandte Chemie·Aaron M Kushner, Zhibin Guan
Aug 3, 2010·Chemical Society Reviews·Jorge A FallasJeffrey D Hartgerink
Aug 1, 2012·Macromolecular Rapid Communications·Fang WeiJeffrey D Hartgerink
Dec 22, 2010·Chemical Communications : Chem Comm·Cillian ByrneWeng C Chan
Jan 21, 2021·Bioengineering·Yujia Xu, Michele Kirchner
Nov 9, 2007·Journal of the American Chemical Society·Varun Gauba, Jeffrey D Hartgerink
Feb 14, 2007·Journal of the American Chemical Society·Varun Gauba, Jeffrey D Hartgerink

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