Rapid synthesis of three-dimensional sulfur-doped porous graphene via solid-state microwave irradiation for protein removal in plasma sample pretreatment

Talanta
Fan LiZhining Xia

Abstract

In this work, we prepared three-dimensional sulfur-doped porous graphene (3D-SPG) via solid-state microwave method and first introduced it to plasma sample pretreatment as adsorbent for the removal of proteins. The efficient heating effect of solid-state microwave irradiation endowed the as-prepared 3D-SPG with large specific surface area, porous structures and sulfur-doped conjugated π electron surface, thus producing an outstanding adsorbent for proteins adsorption. The adsorption behavior of 3D-SPG towards proteins was explored using bovine serum albumin (BSA) as the model protein and several kinetic models and isotherm models were employed to describe the adsorption process. The results indicated that BSA was adsorbed onto 3D-SPG in a monolayer manner with high adsorption capacity, and chemisorption and intraparticle diffusion was the rate-controlling step in proteins adsorption process. By applying 3D-SPG as adsorbent to remove proteins in real rat plasma, we found that 3D-SPG solid phase extraction (SPE) gained exceedingly high protein removal efficiency compared with other plasma pretreatment methods, suggesting that 3D-SPG SPE could effectively prevent the deterioration of column performance and decrease the interferenc...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.