PMID: 22572179Jan 1, 2010Paper

Rapid three-dimensional analysis of renal biopsy sections by low vacuum scanning electron microscopy

Archives of Histology and Cytology
Sumire InagaTomonori Naguro

Abstract

Renal biopsy paraffin sections were examined by low vacuum scanning electron microscopy (LVSEM) in the backscattered electron (BSE) mode, a novel method for rapid pathological analysis which allowed detailed and efficient three-dimensional observations of glomeruli. Renal samples that had been already diagnosed by light microscopy (LM) as exhibiting IgA nephropathy, minor glomerular abnormalities, and membranous glomerulonephritis (GN) were rapidly processed in the present study. Unstained paraffin sections of biopsy samples on glass slides were deparaffinized, stained with platinum blue (Pt-blue) or periodic acid silver-methenamine (PAM), and directly observed with a LVSEM. Overviews of whole sections and detailed observations of individual glomeruli were immediately performed at arbitrary magnifications between ×50 to ×18,000. Cut surface views and surface views of glomeruli were demonstrated at the same time. On Pt-blue-stained sections, podocytes, endothelia, mesangium, and glomerular basement membranes (GBMs) could be distinguished due to the different yields of BSE signals, and pathological features were investigated in every sample. The abnormal surface appearances of podocytes with foot processes and the varying thickne...Continue Reading

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Jun 15, 2007·Archives of Histology and Cytology·Sumire InagaTomonori Naguro

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Citations

Aug 31, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hideya KawasakiTakahiko Hariyama
Oct 31, 2021·Nature Protocols·Tariq Sami HaddadIris Nagtegaal

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