Abstract
Renal biopsy paraffin sections were examined by low vacuum scanning electron microscopy (LVSEM) in the backscattered electron (BSE) mode, a novel method for rapid pathological analysis which allowed detailed and efficient three-dimensional observations of glomeruli. Renal samples that had been already diagnosed by light microscopy (LM) as exhibiting IgA nephropathy, minor glomerular abnormalities, and membranous glomerulonephritis (GN) were rapidly processed in the present study. Unstained paraffin sections of biopsy samples on glass slides were deparaffinized, stained with platinum blue (Pt-blue) or periodic acid silver-methenamine (PAM), and directly observed with a LVSEM. Overviews of whole sections and detailed observations of individual glomeruli were immediately performed at arbitrary magnifications between ×50 to ×18,000. Cut surface views and surface views of glomeruli were demonstrated at the same time. On Pt-blue-stained sections, podocytes, endothelia, mesangium, and glomerular basement membranes (GBMs) could be distinguished due to the different yields of BSE signals, and pathological features were investigated in every sample. The abnormal surface appearances of podocytes with foot processes and the varying thickne...Continue Reading
References
Jul 1, 1989·Cell and Tissue Research·I W GibsonG B Lindop
Jan 1, 1989·Nephron·S NishimuraZ Ota
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·N Weidner, W B Lorentz
Jan 1, 1986·Ultrastructural Pathology·S M Bonsib, S B Plattner
Apr 1, 1985·Kidney International·S M Bonsib
Nov 1, 1985·Human Pathology·S M Bonsib
Jan 1, 1973·Kidney International·J V Johannessen
Jan 1, 1984·Ultrastructural Pathology·S M Bonsib
Nov 1, 1980·The Histochemical Journal·G M Newcomb, A Boyde
Jul 1, 1983·Renal Physiology·E C Carlson, S N Chatterjee
Aug 1, 1982·Kidney International·R P SwainS K Ainsworth
Jan 1, 1980·Renal Physiology·E C Carlson, M C Kenney
Feb 1, 1982·Journal of Morphology·E C Carlson, M C Kenney
Jul 1, 1982·Pathology·W NgH K Ngai
Jan 1, 1993·Virchows Archiv. A, Pathological Anatomy and Histopathology·W Min, N Yamanaka
Dec 23, 1998·Archives of Histology and Cytology·K TanakaS Saito
Mar 15, 2003·Environmental Science & Technology·Satoshi Utsunomiya, Rodney C Ewing
Apr 12, 2003·Archives of Histology and Cytology·Sumire InagaAkihiro Iino
Jul 13, 2004·Applied Microbiology and Biotechnology·Y HatadaK Horikoshi
Oct 7, 2004·Journal of the American Society of Nephrology : JASN·Anne-Tiina LahdenkariHannu Jalanko
Jun 15, 2007·Archives of Histology and Cytology·Sumire InagaTomonori Naguro
Dec 17, 2009·Archives of Histology and Cytology·Sumire InagaTomonori Naguro
Citations
Jul 25, 2014·Clinical and Experimental Nephrology·Yukinari MasudaAkira Shimizu
Aug 26, 2014·Biomedical Research·Shinichi OkadaSusumu Kanzaki
Aug 31, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hideya KawasakiTakahiko Hariyama
Jan 29, 2019·Clinical and Experimental Nephrology·Yusuke KajimotoAkira Shimizu
Oct 31, 2014·Biomedical Research·Shinichi OkadaSusumu Kanzaki
Mar 10, 2016·Microscopy·Tetsuya YanoMotoji Sawabe
Oct 8, 2020·Medical Molecular Morphology·Shumpei MukaiKazuho Honda
Apr 17, 2021·BMC Nephrology·Shinya TaguchiShuzo Kobayashi
May 21, 2021·KI Reports·Takuya FujimaruEisei Sohara
Oct 31, 2021·Nature Protocols·Tariq Sami HaddadIris Nagtegaal
Mar 13, 2021·Case Reports in Nephrology and Dialysis·Maria YoshidaTakao Masaki