Oct 26, 2018

Rapid Tilt-Series Acquisition for Electron Cryotomography

BioRxiv : the Preprint Server for Biology
Georges ChreifiGrant J Jensen

Abstract

Using a new Titan Krios stage equipped with a single-axis holder, we developed two methods to accelerate the collection of tilt-series. We demonstrate a continuous-tilting method that can record a tilt-series in seconds (about 100x faster than current methods), but with loss of details finer than ~4 nm. We also demonstrate a fast-incremental method that can record a tilt-series about 10x faster than current methods and with similar resolution. We characterize the utility of both methods in real biological electron cryotomography workflows. We identify opportunities for further improvements in hardware and software and speculate on the impact such advances could have on structural biology.

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Mentioned in this Paper

Axis
Electron Microscopy
Computer Software
Tilt (brand of fungicide)

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