Rapid treatment of full-thickness skin loss using ovine tendon collagen type I scaffold with skin cells

Journal of Tissue Engineering and Regenerative Medicine
Fauzi Mh BusraShiplu Roy Chowdhury

Abstract

The full-thickness skin wound is a common skin complication affecting millions of people worldwide. Delayed treatment of this condition causes the loss of skin function and integrity that could lead to the development of chronic wounds or even death. This study was aimed to develop a rapid wound treatment modality using ovine tendon collagen type I (OTC-I) bio-scaffold with or without noncultured skin cells. Genipin (GNP) and carbodiimide (EDC) were used to cross-link OTC-I scaffold to improve the mechanical strength of the bio-scaffold. The physicochemical, biomechanical, biodegradation, biocompatibility, and immunogenicity properties of OTC-I scaffolds were investigated. The efficacy of this treatment approach was evaluated in an in vivo skin wound model. The results demonstrated that GNP cross-linked OTC-I scaffold (OTC-I_GNP) had better physicochemical and mechanical properties compared with EDC cross-linked OTC-I scaffold (OTC-I_EDC) and noncross-link OTC-I scaffold (OTC-I_NC). OTC-I_GNP and OTC-I_NC demonstrated no toxic effect on cells as it promoted higher cell attachment and proliferation of both primary human epidermal keratinocytes and human dermal fibroblasts compared with OTC-I_EDC. Both OTC-I_GNP and OTC-I_NC exhi...Continue Reading

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Citations

Apr 9, 2020·Frontiers in Bioengineering and Biotechnology·Fengxuan HanBin Li
Jun 18, 2020·International Journal of Environmental Research and Public Health·Nusaibah SallehuddinMh Busra Fauzi
Nov 19, 2020·Polymers·Mior Muhammad Amirul ArifMohd Heikal Mohd Yunus
Jul 1, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Liangyi WeiWenguo Cui
Aug 28, 2021·Biomedicines·Izzat Zulkiflee, Mh Busra Fauzi

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