Rapid Two-Millisecond Interrogation of Electrochemical, Aptamer-Based Sensor Response Using Intermittent Pulse Amperometry

ACS Sensors
Mirelis Santos-CancelRyan J White

Abstract

In this manuscript, we employ the technique intermittent pulse amperometry (IPA) to interrogate equilibrium and kinetic target binding to the surface of electrochemical, aptamer-based (E-AB) sensors, achieving as fast as 2 ms time resolution. E-AB sensors comprise an electrode surface modified with a flexible nucleic acid aptamer tethered at the 3'-terminus with a redox-active molecule. The introduction of a target changes the conformation and flexibility of the nucleic acid, which alters the charge transfer rate of the appended redox molecule. Typically, changes in charge transfer rate within this class of sensor are monitored via voltammetric methods. Here, we demonstrate that the use of IPA enables the detection of changes in charge transfer rates (i.e., current) at times <100 μs after the application of a potential pulse. Changes in sensor current are quantitatively related to target analyte concentration and can be used to create binding isotherms. Furthermore, the application of IPA enables rapid probing of the electrochemical surface with a time resolution equivalent to as low as twice the applied potential pulse width, not previously demonstrated with traditional voltammetric techniques employed with E-AB sensors (alter...Continue Reading

References

Oct 1, 2004·Analytical Chemistry·Michael L A V HeienR Mark Wightman
Aug 25, 2005·Nature Methods·Eugene V Mosharov, David Sulzer
Mar 8, 2008·Journal of the American Chemical Society·Elena E FerapontovaKurt V Gothelf
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Apr 14, 2016·Annual Review of Analytical Chemistry·Lauren R Schoukroun-BarnesRyan J White

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Citations

Dec 14, 2018·Chemical Communications : Chem Comm·Juan SongZhenyu Lin
Mar 10, 2021·Nature Chemistry·Jagotamoy DasShana O Kelley
Jul 28, 2021·Annual Review of Analytical Chemistry·Kenneth R WehmeyerWilliam R Heineman
Sep 23, 2020·Analytical Chemistry·Alex M DownsPhilippe Dauphin-Ducharme
Aug 30, 2019·Analytical Chemistry·Samuel D CurtisNetzahualcóyotl Arroyo-Currás

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