Rapidly increased neuronal mitochondrial biogenesis after hypoxic-ischemic brain injury.

Stroke; a Journal of Cerebral Circulation
Wei YinJun Chen

Abstract

Mitochondrial biogenesis is regulated through the coordinated actions of both nuclear and mitochondrial genomes to ensure that the organelles are replenished on a regular basis. This highly regulated process has been well defined in skeletal and heart muscle, but its role in neuronal cells, particularly when under stress or injury, is not well understood. In this study, we report for the first time rapidly increased mitochondrial biogenesis in a rat model of neonatal hypoxic/ischemic brain injury (H-I). Postnatal day 7 rats were subjected to H-I induced by unilateral carotid artery ligation followed by 2.5 hours of hypoxia. The relative amount of brain mitochondrial DNA (mtDNA) was measured semiquantitatively using long fragment PCR at various time points after H-I. HSP60 and COXIV proteins were detected by Western blot. Expression of three genes critical for the transcriptional regulation of mitochondrial biogenesis, peroxisome proliferator-activated receptor coactivator-1 (PGC-1), nuclear respiratory factor-1 (NRF-1), and mitochondrial transcription factor A (TFAM), were examined by Western blot and RT-PCR. Brain mtDNA content was markedly increased 6 hours after H-I, and continued to increase up to 24 hours after H-I. Parall...Continue Reading

References

Feb 1, 1981·Annals of Neurology·J E RiceJ B Brierley
Mar 25, 2000·Journal of Cellular Biochemistry·Z ZengT Tong
Nov 10, 2000·Biochimica Et Biophysica Acta·K H KainN K Herzog
Oct 6, 2001·Stroke; a Journal of Cerebral Circulation·H ChenC Y Hsu
Nov 20, 2002·Circulation·S Gupta, A A Knowlton
Jan 25, 2003·Medicine and Science in Sports and Exercise·David A HoodArne A Rungi
Sep 21, 2004·Journal of Bioenergetics and Biomembranes·Henrik Hagberg
Nov 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Enzo NisoliEmilio Clementi
Sep 24, 2005·Annals of the New York Academy of Sciences·Nicolas G BazanKasie Cole-Edwards
Oct 19, 2005·The Journal of Biological Chemistry·Claude A Piantadosi, Hagir B Suliman
Nov 22, 2005·Neuroscience·D R GutsaevaC A Piantadosi
Dec 6, 2005·Journal of Cellular Biochemistry·Richard C Scarpulla
Jan 31, 2006·Free Radical Biology & Medicine·Klas Blomgren, Henrik Hagberg
Feb 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·G López-LluchR de Cabo
Mar 27, 2007·Annals of the New York Academy of Sciences·Carlo Bertoni-FreddariClara De Angelis
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Biplab Dasgupta, Jeffrey Milbrandt

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Citations

Jan 25, 2014·Lancet Neurology·Henrik HagbergClaire Thornton
Jan 28, 2010·The Journal of Pharmacology and Experimental Therapeutics·Jason A FunkRick G Schnellmann
Dec 14, 2011·American Journal of Physiology. Renal Physiology·Jason A Funk, Rick G Schnellmann
Nov 11, 2011·International Journal of Molecular Sciences·Shang-Der ChenYao-Chung Chuang
Oct 26, 2012·ISRN Neurology·Carina Mallard
Dec 18, 2013·Apoptosis : an International Journal on Programmed Cell Death·Dongwook LeeWon-Kyu Ju
Feb 22, 2012·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Catherine I RoussetHenrik Hagberg
Nov 12, 2014·International Journal of Molecular Sciences·Xiaowan WangShinghua Ding
Oct 10, 2009·Journal of Neuropathology and Experimental Neurology·Amanda M SnyderJames R Connor
Nov 4, 2011·Trends in Endocrinology and Metabolism : TEM·Ian S Patten, Zolt Arany
Sep 11, 2010·Journal of the Neurological Sciences·Katalin SasLászló Vécsei
Aug 6, 2010·Progress in Neurobiology·R Anne StetlerJun Chen
Oct 25, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R Anne StetlerJun Chen
Mar 29, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Miguel A Perez-PinzonGary Fiskum
Apr 6, 2016·Neural Plasticity·Eridan Rocha-Ferreira, Mariya Hristova
Jan 20, 2015·International Journal of Stroke : Official Journal of the International Stroke Society·Jilin Bai, Patrick D Lyden
Aug 1, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jeroen PoelsKoenraad K Norga
Oct 19, 2011·Journal of Neurochemistry·Rachel WilliamsSteven M LeVine

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