Rapidly screening of α-glucosidase inhibitors from Dioscorea opposita Thunb. peel based on rGO@Fe3 O4 nanocomposites microreactor

Journal of Enzyme Inhibition and Medicinal Chemistry
Songsong ZhangXiuhua Liu

Abstract

Present study aimed to immobilise the α-glucosidase on suitable supports to construct enzymatic microreactors and their subsequent applicability in efficient inhibitor screening from the Chinese Yam (Dioscorea opposita Thunb.) peel. A type of lamellar and porous composites (rGO@Fe3O4) were synthesised with a facile one-step solvothermal method and employed as carriers to construct enzymatic microreactors for screening α-glucosidase ligand from the Chinese Yam peel in league with the high performance liquid chromatography and mass spectrometry (HPLC-MS). The immobilisation amount of α-glucosidase on rGO@Fe3O4 under the optimised conditions was about 40 μg α-glucosidase/mg carriers. Furthermore, the binding capacities of screened inhibitors, 2,4-dimethoxy-6,7-dihydroxyphenanthrene and batatasin I, were 35.6 and 68.2%, respectively. Hence, considering their high screening efficiency and excellent magnetic separation ability, these as-prepared nanocomposite consisting of rGO and Fe3O4 may be potential supports for the enzyme (such as α-glucosidase) immobilisation for rapid α-glucosidase inhibitors screening from the diverse nature resources.

References

Aug 1, 1997·Bioscience, Biotechnology, and Biochemistry·S Chiba
Feb 12, 2005·Preparative Biochemistry & Biotechnology·Seçil OnalFigen Zihnioğlu
May 20, 2009·Biochemical and Biophysical Research Communications·Sheng-Yang WangJu-Chien Cheng
Jun 26, 2013·Food Chemistry·Kannan R R RengasamyJohannes Van Staden
Oct 21, 2014·Journal of Agricultural and Food Chemistry·Yuan-Ting ZhuXun Liao

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Citations

Aug 7, 2021·Journal of Pharmaceutical and Biomedical Analysis·Isabela Abreu Trindade XimenesMarcela Cristina de Moraes

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Methods Mentioned

BETA
X-ray

Software Mentioned

MPMS3

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