PMID: 11329617May 1, 2001Paper

Ras-induced colony formation and anchorage-independent growth inhibited by elevated expression of Puralpha in NIH3T3 cells

Journal of Cellular Biochemistry
Sharon M Barr, E M Johnson

Abstract

Levels of Puralpha, a conserved, sequence-specific single-stranded DNA and RNA binding protein, fluctuate during the cell cycle, declining at the onset of S-phase and peaking at mitosis. In early G1 Puralpha is associated with the hypophosphorylated form of the retinoblastoma protein, Rb. Microinjection of purified Puralpha into NIH3T3 cells arrests the cell cycle at either G1/S or G2/M checkpoints with distinct morphological consequences. Here we ask whether expression of Puralpha can affect colony formation and anchorage-independent growth in ras-transformed NIH3T3 cells. Two to five-fold elevated levels of Puralpha in stably-transfected cell lines retard entry into and progression through S phase in both ras-transformed and non-transformed cells. Puralpha significantly inhibits colony formation by ras-transformed cells but not by non-transformed cells. In addition, cells transfected to express Puralpha formed only about 1/5 the number of large colonies in soft agar as control-transfected cells, demonstrating a marked inhibition of anchorage-independent growth by Puralpha. Biochemical analysis of nuclear and cytoplasmic Puralpha proteins and confocal microscopic analysis of Puralpha location indicate that access of Puralpha t...Continue Reading

References

Dec 11, 1992·Nucleic Acids Research·Y HeraultD Michel
Feb 1, 1987·The Journal of Cell Biology·G PipernoX J Chang
Mar 1, 1986·Experimental Cell Research·D L Spector, H C Smith
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O Danos, R C Mulligan
Oct 6, 1995·The Journal of Biological Chemistry·C AlbaneseR G Pestell
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W S PearD Baltimore
May 15, 1995·Genes & Development·C J Sherr, J M Roberts
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M M Le BeauC A Westbrook
Dec 15, 1995·The Journal of Biological Chemistry·J C ReddyJ D Licht
Jul 1, 1996·Trends in Biochemical Sciences·M Rechsteiner, S W Rogers
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·C P KrachmarovE M Johnson
Oct 31, 1996·Annals of the New York Academy of Sciences·T OsugiN Miki
Jul 22, 1998·The EMBO Journal·A HagtingJ Pines
Jan 20, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·R PalmqvistG Landberg

❮ Previous
Next ❯

Citations

Oct 20, 2011·Human Molecular Genetics·Suvi HokkanenJochen Herms
Feb 15, 2005·Biochemical and Biophysical Research Communications·Hong LiuEdward M Johnson
May 15, 2007·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Fangli LiuPu Li
Dec 19, 2001·Journal of Cellular Physiology·N DarbinianK Khalili
Sep 21, 2002·Microscopy Research and Technique·Dianne C Daniel
Sep 29, 2012·Journal of Cellular Physiology·Edward M JohnsonJennifer Gordon
Jun 3, 2006·Journal of Cellular Biochemistry·Nune DarbinianKamel Khalili
Mar 22, 2007·British Journal of Cancer·C E S ComstockK E Knudsen
Nov 24, 2006·The Journal of Biological Chemistry·Jon E RamseyRobert J Kelm
Dec 19, 2020·Biochemical and Biophysical Research Communications·Chu-Jen KuoYi-Chao Lee
Feb 10, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Margaret J WortmanDianne C Daniel
Mar 22, 2005·Biochimica Et Biophysica Acta·Margaret J WortmanAndrew D Bergemann
Oct 7, 2004·Developmental Biology·William Todd PenberthyShuo Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.