PMID: 9431806Feb 12, 1998Paper

Ras is an essential component for notochord formation during ascidian embryogenesis

Mechanisms of Development
Y Nakatani, Hiroki Nishida

Abstract

In ascidian embryos, inductive interactions are necessary for the fate specification of notochord cells. Previous studies have shown that notochord induction occurs at the 32-cell stage and that basic fibroblast growth factor (bFGF) has notochord-inducing activity in ascidian embryos. In vertebrate, it is known that bFGF receptors have tyrosine kinase domain and the signaling pathway is mediated by the small-GTP binding protein, Ras. To study the role of Ras in ascidian embryos, we injected dominant negative Ras (RasN17) into fertilized eggs. RasN17 inhibited the formation of notochord, suggesting that the Ras signaling pathway is involved in signal transduction in the induction of notochord cells. When the presumptive-notochord (A6.2) blastomere was co-isolated with the inducer (A6.1) blastomere and then RasN17 was injected into the A6.2 blastomere, notochord differentiation was suppressed. The presumptive-notochord blastomeres injected with RasN17 were treated with bFGF. Many of them failed to develop notochord-specific features. Next, we examined the effect of injecting constitutively active Ras (RasV12) into the A6.2 blastomeres. However, microinjection of RasV12 into these cells did not bypass notochord induction. These re...Continue Reading

References

May 21, 1992·Nature·M Whitman, D A Melton
Apr 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·T Nishikata, N Satoh
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H Okado, K Takahashi
Apr 1, 1989·Developmental Biology·H Nishida, N Satoh
May 1, 1989·The Journal of Experimental Zoology·J R Whittaker, T H Meedel
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·L M GravesD Kimelman
Feb 16, 1995·Nature·T Pawson
Nov 1, 1994·Trends in Biochemical Sciences·M A Lemmon, J Schlessinger
Nov 1, 1994·Developmental Biology·Y Nakatani, H Nishida
Jun 1, 1994·Developmental Biology·R S HartleyJ L Maller
Jan 1, 1993·Annual Review of Biochemistry·D R Lowy, B M Willumsen
Mar 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M J KARNOVSKY, L ROOTS

❮ Previous
Next ❯

Citations

Jul 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hiroki Nishida
Sep 4, 1998·Development, Growth & Differentiation·H Takahashi, N Satoh
Apr 4, 2001·Development, Growth & Differentiation·M ShimadaH Yokosawa
Sep 29, 2001·Development, Growth & Differentiation·G J Kim, H Nishida
Mar 13, 2003·Development, Growth & Differentiation·Hiroki Nishida
Dec 17, 2002·Developmental Biology·P LemaireC Hudson
Oct 7, 2006·Developmental Biology·Hitoyoshi Yasuo, Clare Hudson
Jun 20, 2006·Current Opinion in Genetics & Development·Edwin MunroPatrick Lemaire
Dec 2, 2011·Trends in Cell Biology·Lance A Davidson
Nov 10, 2016·Development·Ignacio A Navarrete, Michael Levine
May 15, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Di Jiang, William C Smith
Jun 24, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hiroki Nishida

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.