Rat abdominal aorta stenting: a new and reliable small animal model for in-stent restenosis

Journal of Vascular Research
Bas LangeveldWiek H van Gilst

Abstract

A high throughput animal model may enhance pathophysiological studies to mechanisms of in-stent restenosis (ISR). More and appropriate antibodies and transgenic and knockout strains are available in rats. Consequently, a model for ISR in the rat would be convenient for pathobiological studies. Here we present the full characteristics of a rat ISR model suitable for high throughput stent research. The abdominal aorta of rats was separated from surrounding tissue and a BeStenttrade mark 2 or a Cyphertrade mark sirolimus-eluting stent was locally inserted. After 1, 3, 7, 28 and 56 days, the aortas were harvested, fixed, embedded and cut. Morphometric analysis was performed and inflammation scored. The neointimal area increased to a maximum after 28 days (0.55 +/- 0.08 mm(2)). Subsequently, the neointimal area slightly decreased. The injury score and the neointimal area were linearly correlated (r = 0.85, p < 0.01). Thrombus formation was present after 1 day. Leukocyte adherence was evident after 1 day, maximal after 3 days (93 +/- 21 cells/section) and decreased thereafter. The inflammation score increased after 3 days to a maximum after 7 days (1.37 +/- 0.06) and declined thereafter. After 28 days the Cypher sirolimus-eluting ste...Continue Reading

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