Rat adipose ob mRNA levels in states of altered circulating glucose and insulin

Biochemical and Biophysical Research Communications
W I SivitzP Donohoue

Abstract

We measured levels of ob mRNA in epididymal fat pads of rats exposed to manipulations designed to alter circulating insulin and glycemia. Changes in ob mRNA were compared to alterations in GLUT-4 glucose transporter mRNA, which is known to be regulated under these conditions. 48 h fasting decreased GLUT-4 mRNA to 23% of control with restoration beginning by 6 h refeeding and full restoration at 24 h. In contrast, ob mRNA decreased less markedly to 47% of control with only partial restoration by 24 h. Two days of streptozocin (STZ)-diabetes (glucose > 400 mg/100 ml) decreased GLUT-4 mRNA to 8% of control with restoration by two days of S.C. insulin. In contrast, ob mRNA decreased to 42% of control and was not restored by insulin. Six days of insulin administration to normal rats under conditions of ad lib. feeding, but without otherwise preventing the blood glucose from decreasing, resulted in no significant change in levels of either ob or GLUT-4 mRNA.

Citations

May 20, 1998·Metabolism: Clinical and Experimental·W I SivitzR L Leibel
Jan 24, 1998·Metabolism: Clinical and Experimental·K WalderG R Collier
Oct 12, 2010·Journal of Physiology and Biochemistry·Mohamed D MorsyOsama M Osman
Aug 1, 1997·Endocrinology·W I SivitzW G Haynes
Mar 10, 1999·The American Journal of Physiology·J Y KhanS U Devaskar
Oct 12, 1999·The American Journal of Physiology·W I SivitzW G Haynes
Apr 6, 2002·American Journal of Physiology. Endocrinology and Metabolism·Chia-Yu LinB Douglas White
Sep 25, 1998·Medizinische Klinik·C SpitzwegA E Heufelder
Oct 12, 2000·Biochemical and Biophysical Research Communications·X CasabiellF F Casanueva
Jan 1, 1997·Peptides·H C FehmannB Göke

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