Rat aortic smooth muscle cells cultured on hydroxyapatite differentiate into osteoblast-like cells via BMP-2-SMAD-5 pathway

Calcified Tissue International
Pranjal Nahar-GohadNaren R Vyavahare

Abstract

Vascular calcification is an important pathological condition associated with increased risk of cardiovascular mortality. Hydroxyapatite (HA) found in such deposits is the same polymorph of calcium (Ca) found in bone, indicating calcification may involve mechanisms akin to bone formation. Vascular smooth muscle cells (Vsmcs) have been shown to undergo phenotypic change to osteoblast-like cells. However, the mechanisms underlying this phenotypic change are unclear, and whether the stimulus to become osteogenic is a result of loss of mineralization inhibitors or early mineral deposits is not known. Our aim in this study is to identify mechanisms and signal transduction pathways that cause differentiation of Vsmcs into osteoblast-like cells in the presence of HA. We first characterized vascular origin of Vsmcs by studying the expression of smooth muscle cell markers: myosin heavy chain and smooth muscle actin along with SM22α at both mRNA and protein levels. Vsmcs grown on HA exhibited progressive change in cellular morphology at 3-, 7-, and 14-day time points. Culturing of Vsmcs on HA disc resulted in decrease in media Ca levels and increased expression of Ca-sensing receptor (CaSR) on Vsmcs resulting in upregulation of intracell...Continue Reading

References

Apr 1, 1988·Experimental Cell Research·I A SilverD J Etherington
Oct 1, 1994·The Journal of Clinical Investigation·L A FitzpatrickR T Ingram
May 1, 1994·The Journal of Clinical Investigation·K E WatsonL L Demer
Apr 1, 1993·The Journal of Clinical Investigation·K BoströmL L Demer
Sep 22, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·P A PriceM K Williamson
Oct 12, 2000·Journal of the American College of Cardiology·Y AradA D Guerci
Dec 14, 2001·The Journal of Biological Chemistry·Amina F ZebboudjKristina Boström
Jan 15, 2002·Journal of the American College of Cardiology·Roberto WayhsPaolo Raggi
Aug 26, 2003·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Gérard M LondonHasan Adda
Oct 28, 2003·The Journal of Biological Chemistry·Paul A PriceTruclinh N Than
Feb 26, 2004·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·William G GoodmanUNKNOWN Vascular Calcification Work Group
Apr 16, 2004·Endocrinology·Naibedya ChattopadhyayEdward M Brown
Jul 23, 2005·Circulation Research·Keith A HruskaGeorges Saab
Sep 14, 2006·Calcified Tissue International·Jinxi WangMelvin J Glimcher
Oct 4, 2006·Nephrology·Diane Proudfoot, Catherine M Shanahan
Nov 11, 2006·Circulation Research·Rebecca C JohnsonJoseph Loscalzo
Dec 2, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dinithi PeirisR John MacLeod
Jan 27, 2007·Circulation Research·Georgina D M CollettAnn E Canfield
Jul 16, 2008·Bioorganic & Medicinal Chemistry Letters·Gregory D CunyRandall T Peterson
May 7, 2010·Clinical Science·Adrian CovicDavid J Goldsmith
Mar 17, 2011·Advances in Chronic Kidney Disease·Wei Ling LauCecilia M Giachelli
Nov 10, 2012·Journal of the American Society of Nephrology : JASN·Rukshana ShroffCatherine Shanahan

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Citations

Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Suren P UswattaAmbalangodage C Jayasuriya
Jun 22, 2019·Journal of Cellular Physiology·Ling-Hong HuangBao-Song Gui
Jun 5, 2019·Scientific Reports·Andre P MarshallRaul J Guzman
May 6, 2017·BioMed Research International·Yue GuXiaoxue Bai
May 11, 2021·Frontiers in Cardiovascular Medicine·Wenhong JiangXiao Qin

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