Rat aortic smooth muscle cells expressing charybdotoxin-sensitive potassium channels exhibit enhanced proliferative responses

Clinical and Experimental Pharmacology & Physiology
C B NeylonA Bobik

Abstract

1. The relationship between the expression of potassium (K+) channels and the growth properties of cultured vascular smooth muscle cells was examined. 2. Two groups of cells having different proliferative rates were cultured from the Wistar-Kyoto rat aorta. One group of cells, derived from early passages (3-5), proliferated with a cell doubling time of 2.41 days. A second group of cells, derived from late passages (> 12), proliferated at a higher rate (cell doubling time, 0.61 days). 3. Exposure of the early passaged cells to endothelin-1 (0.1 mumol/L) induced membrane depolarization. In contrast, exposure of the late passage cells to endothelin-1 (0.1 mumol/L) evoked a rapid hyperpolarization. The hyperpolarization in the late passage cells was blocked by charybdotoxin (20 nmol/L), an inhibitor of the large-conductance Calcium (Ca)-activated K+ channel. 4. The authors conclude that rapidly proliferating vascular smooth muscle cells express enhanced activity of Ca-activated K+ channels causing marked alterations in the electrical properties of the cells. It is therefore suggested that the reported increase in Ca-activated K+ channel activity in the aortae of hypertensive rats is likely to be associated with the increased prolif...Continue Reading

References

Oct 1, 1990·Physiological Reviews·M J Mulvany, C Aalkjaer
Oct 1, 1990·The American Journal of Physiology·C B NeylonR F Irvine
Nov 11, 1986·The American Journal of Physiology·P J LittleA Bobik
Mar 1, 1987·Clinical and Experimental Pharmacology & Physiology·A BobikP J Little
Dec 30, 1988·Biochemical and Biophysical Research Communications·C Van RenterghemM Lazdunski
Apr 1, 1982·Physiological Reviews·B Folkow

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Citations

Aug 7, 1998·Journal of Pharmacological and Toxicological Methods·C HerrmannK Lang
Apr 20, 2001·The International Journal of Biochemistry & Cell Biology·M FaehlingJ Waltenberger
Dec 1, 1995·British Journal of Pharmacology·F C HallidayA M Gurney
Jun 23, 2007·American Journal of Respiratory Cell and Molecular Biology·Malcolm C ShepherdAlastair G Stewart
Apr 16, 2011·American Journal of Respiratory Cell and Molecular Biology·Zhifei ShaoDevendra K Agrawal
Apr 6, 2011·Journal of Molecular and Cellular Cardiology·Xing-Li SuXiu-Ling Deng
Dec 6, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Li-Mei ZhaoXiu-Ling Deng
Jun 27, 2006·American Journal of Physiology. Heart and Circulatory Physiology·D L TharpD K Bowles
Dec 18, 2013·Nature Reviews. Cancer·Luis A Pardo, Walter Stühmer
Aug 5, 2017·Frontiers in Physiology·Bob Shih-Liang LeeKirk L Hamilton

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