Rat arterial smooth muscle devoid of ryanodine receptor function: effects on cellular Ca(2+) handling

British Journal of Pharmacology
K DrejaP Hellstrand

Abstract

The roles of intracellular Ca(2+) stores and ryanodine (Ry) receptors for vascular Ca(2+) homeostasis and viability were investigated in rat tail arterial segments kept in organ culture with Ry (10 - 100 microM) for up to 4 days. Acute exposure to Ry or the non-deactivating ryanodine analogue C(10)-O(eq) glycyl ryanodine (10 microM) eliminated Ca(2+) release responses to caffeine (20 mM) and noradrenaline (NA, 10 microM), whereas responses to NA, but not caffeine, gradually returned to normal within 4 days of exposure to RY: Ry receptor protein was detected on Western blots in arteries cultured either with or without RY: Brief Ca(2+) release events (sparks) were absent after culture with Ry, whereas Ca(2+) waves still occurred. The propagation velocity of waves was equal ( approximately 19 microm s(-1)) in tissue cultured either with or without RY: Inhibition of Ca(2+) accumulation into the sarcoplasmic reticulum (SR) by culture with caffeine (5 mM), cyclopiazonic acid or thapsigargin (both 10 microM) decreased contractility due to Ca(2+)-induced cell damage. In contrast, culture with Ry did not affect contractility. Removal of Ca(2+) from the cytosol following a Ca(2+) load was retarded after Ry culture. Thapsigargin reduced t...Continue Reading

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Citations

Apr 27, 2011·Acta Physiologica·C AalkjærV Matchkov
Mar 27, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Damodaran NarayananJonathan H Jaggar
Aug 13, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Karl DrejaKarl Swärd
Apr 20, 2002·Circulation Research·Karl SwärdPer Hellstrand
Feb 24, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Eun Ji KimSe Hoon Kim
May 1, 2010·Journal of Cellular Physiology·John G McCarronMarnie L Olson
Dec 8, 2005·Canadian Journal of Physiology and Pharmacology·Cheng-Han LeeCornelis van Breemen
Nov 23, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Joseph ZachariaW Gil Wier
Jan 21, 2010·Physiological Reviews·Susan Wray, Theodor Burdyga
Dec 17, 2004·Pharmacological Reviews·Régent LaporteIsmail Laher
Apr 10, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Christine Lamont, W Gil Wier
Jan 29, 2005·British Journal of Pharmacology·Christian Aalkjaer, Holger Nilsson
Dec 13, 2002·American Journal of Physiology. Cell Physiology·George D Thorne, Richard J Paul
Jul 21, 2004·Cell Calcium·George D ThorneRichard J Paul
Oct 31, 2006·Cell Calcium·John G McCarronThomas C Muir

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