PMID: 6171812Oct 1, 1981Paper

Rat basophilic leukemia cell lines defective in phospholipid methyltransferase enzymes, Ca2+ influx, and histamine release: reconstitution by hybridization

Proceedings of the National Academy of Sciences of the United States of America
A McGivneyR P Siraganian

Abstract

Variants of the rat basophilic leukemia (RBL) cell line were isolated and screened for phospholipid methyltransferase I and II activities, enzymes that convert phosphatidylethanolamine to phosphatidylcholine. Two variants were found that had decreased phospholipid methyltransferase enzyme levels and were unable to cause an influx of Ca2+ or release histamine in an IgE-mediated reaction. However, these cells were able to release histamine through an ionophore-induced reaction, indicating that the releasing mechanism distal to the Ca2+ channel was intact. One cell line, 1C1.B1, had low specific activity for phospholipid methyltransferase I. A second variant, 2H3.B6, had reduced phospholipid methyltransferase II activity. Although both variants were unable to incorporate label from [methyl-3H]methionine or [3H]serine into phosphatidylcholine, they were able to incorporate [methyl-3H]choline and myo-[2-3H(N)]inositol into phospholipids. Fusion of the two cell lines and isolation on selective media resulted in the growth of eight independent hybrids. All eight had an increased number of chromosomes and normal phospholipid methyltransferase activities. Stimulation of the hybrids with IgE resulted in CA2+ influx and histamine release....Continue Reading

References

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Citations

Mar 7, 1984·Biochimica Et Biophysica Acta·V PanagiaN S Dhalla
Jul 1, 1989·Brain Research. Brain Research Reviews·F B Meyer
Jun 25, 1984·Biochimica Et Biophysica Acta·S L Pelech, D E Vance
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D L BareisJ Axelrod
Jun 1, 1983·The Journal of Experimental Medicine·N OridaF T Liu
Jan 1, 1980·Pharmacology, Biochemistry, and Behavior·J Axelrod, F Hirata
Mar 31, 2016·Chinese Journal of Natural Medicines·Yong-Xin WangJing Shang
Feb 1, 1985·Journal of Dairy Science·B K VonderhaarT M Horn
Jan 1, 1984·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·D J Triggle

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