PMID: 29762987Feb 1, 2017Paper

Rat bone marrow mesenchymal stem cells promote apoptosis and inhibit proliferation and migration of RSC96 cells

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Yongbin MaJianhua Su

Abstract

To investigate the effect of rat bone marrow mesenchymal stem cells( BMSCs) on the proliferation and migration of rat RSC96 Schwann cells in vitro. BMSCs of Sprague Dawley( SD) rats were isolated by bone marrow cell adherent method. The morphology of the third generation of BMSCs was observed by inverted microscopy. The cell surface antigen makers CD19,CD34,CD73,CD105 were detected by flow cytometry. The third-passage BMSCs differentiated into osteoblasts and adipocytes after exposed to bone-inducing and lipid-inducing media for 3 and 2 weeks,respectively,and then were identified by oil red O and alkaline phosphatase staining. The third generation of BMSCs was co-cultured with RSC96 cells for 24 hours in 24 mm co-culture plate. The cell proliferation and colony formation of RSC96 cells were determined by MTT assay and plate cloning assay. The migration of RSC96 cells was detected by scratch and TranswellTMchamber assay. Bax and Bcl-2 expression levels of RSC96 cells were detect by Western blot analysis. The third generation of BMSCs showed fusiform,spiral arrangement with similar cell size under an inverted microscope. The CD73 and CD105 expression levels of BMSCs were high,however,the CD34 and CD19 were low. BMSCs had large li...Continue Reading

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