Rat brain cortex mitochondria release group II secretory phospholipase A(2) under reduced membrane potential

The Journal of Biological Chemistry
Lara MacchioniGianfrancesco Goracci

Abstract

Activation of brain mitochondrial phospholipase(s) A(2) (PLA(2)) might contribute to cell damage and be involved in neurodegeneration. Despite the potential importance of the phenomenon, the number, identities, and properties of these enzymes are still unknown. Here, we demonstrate that isolated mitochondria from rat brain cortex, incubated in the absence of respiratory substrates, release a Ca(2+)-dependent PLA(2) having biochemical properties characteristic to secreted PLA(2) (sPLA(2)) and immunoreacting with the antibody raised against recombinant type IIA sPLA(2) (sPLA(2)-IIA). Under identical conditions, no release of fumarase in the extramitochondrial medium was observed. The release of sPLA(2) from mitochondria decreases when mitochondria are incubated in the presence of respiratory substrates such as ADP, malate, and pyruvate, which causes an increase of transmembrane potential determined by cytofluorimetric analysis using DiOC(6)(3) as a probe. The treatment of mitochondria with the uncoupler carbonyl cyanide 3-chlorophenylhydrazone slightly enhances sPLA(2) release. The increase of sPLA(2) specific activity after removal of mitochondrial outer membrane indicates that the enzyme is associated with mitoplasts. The mitoc...Continue Reading

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Citations

Aug 28, 2013·Journal of Bioenergetics and Biomembranes·Lara MacchioniLanfranco Corazzi
Mar 20, 2010·Molecular Neurobiology·Gianfrancesco GoracciVincenza Nardicchi
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Feb 2, 2013·Neurochemical Research·Hui YangWei-Yi Ong
Nov 20, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Jing JiHülya Bayır
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Oct 24, 2007·Biochimica Et Biophysica Acta·Vincenza NardicchiGianfrancesco Goracci
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Mar 31, 2010·Molecular and Cellular Biochemistry·Lara MacchioniLanfranco Corazzi

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