PMID: 3214181Dec 1, 1988Paper

Rat brain hexokinase: the hydrophobic N-terminus of the mitochondrially bound enzyme is inserted in the lipid bilayer

Archives of Biochemistry and Biophysics
G C Xie, J E Wilson

Abstract

Mitochondrially bound rat brain hexokinase was labeled with the photoactivatable reagent, 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine. This highly hydrophobic reagent is strongly partitioned into the hydrophobic environment of the membrane core, and thus selectively labels segments of a protein that penetrate this region of the membrane. Labeling of hexokinase was shown to be restricted to the N-terminal region of the molecule. Approximately 80% of the radiolabel was removed by treatment of the enzyme with chymotrypsin, which preferentially cleaves a hydrophobic 9-residue sequence at the extreme N-terminus of the enzyme, and it is considered likely that the remaining 20% was associated with two additional hydrophobic residues, immediately adjacent to this segment but not susceptible to cleavage by chymotrypsin. Labeling of the enzyme was shown to be dependent on maintenance of the association with the membrane. These results are consistent with a model in which binding of hexokinase involves insertion of an 11-residue hydrophobic N-terminal "tail," possibly existing in alpha-helical secondary structure, into the hydrophobic core of the membrane.

References

Jul 1, 1977·Archives of Biochemistry and Biophysics·P L Felgner, J E Wilson
Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Jan 1, 1985·Archives of Biochemistry and Biophysics·P G Polakis, J E Wilson
Jan 1, 1985·Archives of Biochemistry and Biophysics·M Nemat-Gorgani, J E Wilson
Oct 15, 1982·Archives of Biochemistry and Biophysics·M Baijal, J E Wilson
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Aug 1, 1982·Biochemical and Biophysical Research Communications·P G Polakis, J E Wilson

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Citations

Jan 9, 2001·Archives of Biochemistry and Biophysics·M Hashimoto, J E Wilson
Dec 21, 2000·Journal of Colloid and Interface Science·Y KuwaharaR Goto
Aug 7, 2008·Journal of Bioenergetics and Biomembranes·John G Pastorino, Jan B Hoek
Jul 17, 1998·Nature Structural Biology·A M MulichakR M Garavito
Nov 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rachna UjwalJeff Abramson
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D A Schwab, J E Wilson
Jul 10, 2013·The Journal of Biological Chemistry·David J RobertsShigeki Miyamoto
May 10, 2007·The Journal of Biological Chemistry·Syed Mohammad MoinShahid Jameel
Jun 18, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·William A T Summers, Deborah A Court
Nov 13, 2010·PloS One·Eugene WyattHossein Ardehali
Sep 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·AmirAli H TalasazRonald W Davis
Jan 21, 2015·Cell Cycle·Satarupa SenPrasun K Datta
Jan 1, 1990·Archives of Biochemistry and Biophysics·G Xie, J E Wilson
Apr 3, 2014·Physiological Reviews·Ildiko Szabo, Mario Zoratti
Sep 11, 2014·British Journal of Pharmacology·Andrew P HalestrapPhilippe Pasdois
Nov 2, 2016·Oncotarget·Alenoush VartanianGelareh Zadeh
Aug 9, 2016·Journal of Bioenergetics and Biomembranes·Stephen HurstShey-Shing Sheu
May 21, 2003·The Journal of Experimental Biology·John E Wilson
May 1, 1992·Archives of Biochemistry and Biophysics·A Preller, J E Wilson
Mar 30, 2010·Molecular Aspects of Medicine·Varda Shoshan-BarmatzNir Arbel

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