PMID: 6401940Feb 1, 1983Paper

Rat brain interstitial fluid pressure measured with micropipettes

The American Journal of Physiology
H Wiig, R K Reed

Abstract

Interstitial fluid pressure (IFP) in the rat brain has been measured with micropipettes, 2-4 microns in diameter, after trepanation and incision of dura. IFP was compared with cerebrospinal fluid pressure (CSFP) measured by a 0.3-mm (OD) cannula in the lateral ventricle. Control IFP averaged 3.43 +/- 0.65 (SD) mmHg (n = 83) with a corresponding CSFP of 3.53 +/- 0.71 (n = 83). Pretreatment with indomethacin to reduce inflammation during preparation did not affect IFP and CSFP. Infusion of a 20% mannitol solution amounting to 5% of body weight resulted in an average IFP of -5.66 mmHg and CSFP of -4.56 mmHg 20 min after start of the infusion, after which a gradual rise in both pressures was observed. Similar results were obtained after infusion of hypertonic urea. Inflation of a cuff around the rat neck resulted in an immediate and similar rise in IFP and CSFP. Increasing the arterial CO2 partial pressure to 80 mmHg increased IFP to 12-14 mmHg and pial venular pressure to 13-15 mmHg, while CSFP rose to about 10 mmHg. The micropuncture technique for measurement of brain IFP is rapid and practically atraumatic and may be used in a variety of animals.

Citations

Mar 15, 2001·American Journal of Physiology. Heart and Circulatory Physiology·P O IversenK Heyeraas
Nov 15, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Ellen Berggreen, Helge Wiig
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·K J Heyeraas, E Berggreen
Sep 4, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gurjit NagraMiles Johnston

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.