Rat cultured neuronal and glial cells respond differently to toxicity of unconjugated bilirubin

Pediatric Research
Rui F M SilvaDora Brites

Abstract

High levels of unconjugated bilirubin (UCB) can be neurotoxic. Nevertheless, the mechanism of UCB interaction with neural cells is still unknown. This study investigates whether cultured rat neurons and astrocytes respond differently to UCB exposure. UCB toxicity was evaluated by lactate dehydrogenase release, induction of apoptosis, cytoskeleton degeneration, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction, and glutamate uptake. Primary cultures of rat brain astrocytes and neurons were incubated at 37 degrees C with 85.5 microM UCB plus 28.5 microM albumin for 4 h. In assays of glutamate uptake, cells were exposed to 80-120 microM UCB plus 100 microM albumin for 15 min. The results showed that after incubation with 85.5 microM UCB, lactate dehydrogenase release was greater in neurons than in astrocytes (38% versus 14%, p < 0.05). Also, levels of apoptosis were markedly enhanced in neurons (29% versus 19%, p < 0.01). In accordance, neuronal cytoskeleton disassembly was evident during incubation with 85.5 microM UCB, whereas equivalent effects on astrocytes required as much as 171 microM. Conversely, inhibition of MTT metabolism and glutamate uptake by UCB was more pronounced in astrocytes than in ne...Continue Reading

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Citations

Dec 20, 2012·Cell and Tissue Research·Cibelle MarianoMaria Alexandra Brito
Oct 23, 2012·Molecular Neurobiology·Andreia BarateiroDora Brites
Apr 23, 2004·Trends in Molecular Medicine·J Donald OstrowClaudio Tiribelli
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Jun 29, 2011·Molecular and Cellular Neurosciences·Ana Rita VazDora Brites

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