Rat cytomegalovirus major immediate-early enhancer switching results in altered growth characteristics

Journal of Virology
Gordon R SandfordWilliam H Burns

Abstract

It has been hypothesized that the major immediate-early (MIE) enhancer of cytomegalovirus (CMV) is important in determining virus tropism and latency because of its essential role in initiating the cascade of early gene expression necessary for virus replication. Although rat CMV (RCMV) and murine CMV (MCMV) exhibit extreme species specificity in vivo, they differ in their ability to replicate in tissue culture. MCMV can replicate in a rat embryo fibroblast (REF) cell line while RCMV does not grow in murine fibroblasts. The tropism is not due to a block in virus entry into the cell. We have constructed a recombinant RCMV in which the RCMV MIE enhancer has been replaced with that of MCMV. Growth of the recombinant virus in tissue culture remains restricted to rat cells, suggesting that other viral and/or host factors are more important in determining in vitro tropism. Unlike findings using recombinant MCMV in which the human CMV (HCMV) MIE enhancer substitutes for the native one (A. Angulo, M. Messerle, U. H. Koszinowski, and P. Ghazal, J. Virol. 72:8502-8509, 1998), infection with our recombinant virus at a low multiplicity of infection resulted in a substantial decrease in virus replication. This occurred despite comparable or...Continue Reading

References

Dec 1, 1991·Journal of Virology·C E IbanezJ A Nelson
Sep 1, 1988·Virology·W H BurnsG R Sandford
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K Dorsch-HäslerU H Koszinowski
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·K KondoE S Mocarski
Jun 1, 1994·Journal of Virology·E J MintonJ G Sissons
Aug 15, 1996·Virology·G R Sandford, W H Burns
Oct 1, 1996·Genome Research·C A HeidP M Williams
Oct 1, 1996·Genome Research·U E GibsonP M Williams

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Citations

Dec 22, 2007·Medical Microbiology and Immunology·Mark F Stinski, Hiroki Isomura
Apr 8, 2011·Journal of Virology·Mildred Foster-CuevasSebastian Voigt
Jan 1, 2021·International Journal of Molecular Sciences·Michelle A Fisher, Megan L Lloyd

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