Rat Cytomegalovirus Vaccine Prevents Accelerated Chronic Rejection in CMV-Naïve Recipients of Infected Donor Allograft Hearts

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
D N StreblowS L Orloff

Abstract

Cytomegalovirus accelerates transplant vascular sclerosis (TVS) and chronic rejection (CR) in solid organ transplants; however, the mechanisms involved are unclear. We determined the efficacy of a CMV vaccine in preventing CMV-accelerated rat cardiac allograft rejection in naïve recipients of CMV+ donor hearts. F344 donor rats were infected with RCMV 5 days prior to heterotopic cardiac transplantation into CMV-naïve or H2 O2 -inactivated RCMV-vaccinated Lewis recipients. Recipients of RCMV-infected donor hearts rejected at POD59, whereas vaccinated recipients exhibited a significantly prolonged time to rejection-POD97, similar to recipients of uninfected donor hearts (POD108). Although all of the donor hearts were preinfected, the vaccinated recipients had lower graft and PBMC viral loads at POD 7 compared to unvaccinated controls. Adoptive T cell and passive antibody transfers from vaccinated Lewis rats into naïve recipients demonstrate that both T-cell and B-cell arms of the adaptive immune response provide protection against CMV-accelerated rejection. Similar findings were obtained when testing three different adjuvants in passive transfer experiments. We have determined that the timing of the vaccine prior to transplantatio...Continue Reading

References

Jan 1, 1985·Intervirology·C A BruggemanC P van Boven
Dec 1, 1983·Antiviral Research·C A BruggemanC P van Boven
Jan 1, 1993·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·C A Bruggeman
Apr 15, 1997·Transplantation·A T ArmstrongC G Orosz
Jan 1, 1994·Transplant International : Official Journal of the European Society for Organ Transplantation·J H BruningC A Bruggeman
Sep 14, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ajit P Limaye
Dec 24, 2002·Seminars in Respiratory Infections·Ajit P Limaye
Jul 15, 2003·Clinical Microbiology Reviews·Andrea DuchiniPaul J Pockros
Jul 23, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Martin R Zamora
Jan 8, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Raju RadhaMieko Toyoda
Feb 15, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Daniel N StreblowSusan L Orloff
Sep 9, 2005·The Journal of Experimental Medicine·Andrew W SylwesterLouis J Picker
Jan 3, 2006·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mark A JacobsonStuart P Adler
Jan 18, 2007·The Journal of Experimental Medicine·Falk Nimmerjahn, Jeffrey V Ravetch
Apr 20, 2007·Clinical and Vaccine Immunology : CVI·Elizabeth A ReapJeffrey D Chulay
Aug 4, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Robert F Pass
Oct 29, 2010·Journal of Virology·Christine MeyerDaniel N Streblow
Jan 5, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S L OrloffD N Streblow
Oct 4, 2011·Nature Reviews. Microbiology·Stanley A Plotkin, Susan L Plotkin
May 29, 2012·Nature Medicine·Ian J AmannaMark K Slifka
Apr 26, 2013·Vaccine·Paul GriffithsStephanie Bialek
Mar 19, 2014·Methods in Molecular Biology·Suresh B Boppana, William J Britt

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Citations

Jan 10, 2017·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M K NelsenR G Gill
Sep 25, 2018·Expert Review of Vaccines·Don Jeffrey DiamondJohn A Zaia
Dec 14, 2018·Viruses·Christopher C Nguyen, Jeremy P Kamil
Nov 19, 2020·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Nicole N HaeseDaniel N Streblow
Oct 30, 2020·Frontiers in Immunology·Ronald G Gill, Adam L Burrack
Mar 12, 2021·Pharmacological Reviews·Timo W M De GroofMartine J Smit

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Methods Mentioned

BETA
PCR
ELISA
flow cytometry

Clinical Trials Mentioned

NCT00133497

Software Mentioned

GraphPad Prism
Prism

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American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
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