PMID: 6968911Sep 1, 1980Paper

Rat dendritic cells function as accessory cells and control the production of a soluble factor required for mitogenic responses of T lymphocytes

Proceedings of the National Academy of Sciences of the United States of America
W E KlinkertW E Bowers

Abstract

Transformation of T lymphocytes, induced by treatment with periodate or with neuraminidase plus galactose oxidase, requires the participation of accessory cells. Procedures were developed for the fractionation of rat lymph node cells, by which most of the lymphocytes can be recovered as a major population of cells that do not respond to mitogenic stimulation unless accessory cells from a separated minor population are added. Further purification led to a 1000-fold overall increase in accessory activity per cell, with a 50-70% yield. The purest preparations were virtually free of macrophages and contained more than 90% typical dendritic cells. Maximum responses occurred at a ratio of only one dendritic cell per 200 periodate-treated lymphocytes. This evidence thus indicates strongly that in rats, dendritic cells--not macrophages--function as accessory cells. Further, the number of dendritic cells in a preparation governed the magnitude of the mitogenic response and was limiting in the case of unfractionated lymph node cells. In addition, when oxidized with periodate or with neuraminidase plus galactose oxidase, the dendritic cell served as a very potent indirect stimulator of untreated responder lymphocytes. Both functions of th...Continue Reading

References

Mar 15, 1976·Cellular Immunology·D M Chen, G Di Sabato
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R M Steinman, M D Witmer
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C F Beyer, W E Bowers
Jan 1, 1978·Immunological Reviews·U PerssonC I Smith
Feb 1, 1974·The Journal of Experimental Medicine·R M Steinman, Z A Cohn
Jul 1, 1972·The Journal of Experimental Medicine·I GeryB H Waksman
Feb 14, 1980·Nature·E L LarssonA Coutinho

❮ Previous
Next ❯

Citations

Jan 1, 1991·Virchows Archiv. A, Pathological Anatomy and Histopathology·T Wakabayashi, H Onoda
Aug 1, 1982·Immunopharmacology·S E CalvanoG Fernandes
Jan 1, 1982·Veterinary Immunology and Immunopathology·F KristensenS Lazáry
May 1, 1984·Veterinary Immunology and Immunopathology·J Hopkins, I McConnell
Apr 1, 1985·Veterinary Immunology and Immunopathology·E M GoodellW E Bowers
Jan 1, 1990·Neuroscience·J M LawrenceG Raisman
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M C NussenzweigB Gutchinov
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S C KnightJ Clark
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K InabaR M Steinman
Jul 1, 1981·The Journal of Experimental Medicine·M C NussenzweigZ A Cohn
Apr 1, 1982·The Journal of Experimental Medicine·W C Van VoorhisG Kaplan
Jul 1, 1982·The Journal of Experimental Medicine·W E KlinkertW E Bowers
Feb 1, 1983·The Journal of Experimental Medicine·R M SteinmanM C Nussenzweig
Apr 1, 1983·The Journal of Experimental Medicine·J M AustynM A Palladino
May 1, 1983·The Journal of Experimental Medicine·D M SpaldingR M Steinman
Jun 1, 1983·The Journal of Experimental Medicine·C W PughH W Steer
Oct 1, 1983·The Journal of Experimental Medicine·M Kurosaka, M Ziff
Mar 1, 1985·The Journal of Experimental Medicine·G Schuler, R M Steinman
Apr 1, 1986·The Journal of Experimental Medicine·W E Bowers, M R Berkowitz
Jun 1, 1986·Journal of Neuroimmunology·H J Schluesener
Mar 1, 1986·Journal of Neuroimmunology·H J Schluesener, H Lassmann
Nov 1, 1983·The Journal of Experimental Medicine·G H SunshineJ W Kappler
Jan 1, 1988·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M SantamariaJ Peña
Mar 11, 1983·Journal of Immunological Methods·H Wekerle
Nov 30, 1984·Journal of Immunological Methods·W E Bowers, M R Berkowitz
Dec 1, 1982·The American Journal of Anatomy·I Oláh, B Glick
Dec 1, 1984·Immunobiology·W E Bowers, E M Goodell
Dec 1, 1984·Immunobiology·E B Bell
Mar 1, 1982·Immunology Today·M C Nussenzweig, R M Steinman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.