Rat germinal cells require PARP for repair of DNA damage induced by gamma-irradiation and H2O2 treatment

European Journal of Cell Biology
L AtorinoP Quesada

Abstract

The ability of rat germinal cells to recover from genotoxic stress has been investigated using isolated populations of primary spermatocytes and round spermatids. Using a comet assay at pH 10.0 to assess single strand breakage (SSB) in DNA, it was found that a high level of damage was induced by 5 Gy gamma-irradiation and acute exposure to 50 microM H2O2. This damage was effectively repaired during a subsequent recovery period of 1-3 hours culture in vitro but repair was significantly delayed in the presence of the poly(ADP-ribose)polymerase (PARP) inhibitor 3-aminobenzamide (3-ABA). Immunofluorescence detection of PARP with specific antibodies localised the protein to discrete foci within the nucleus of both spermatocytes and spermatids. Poly(ADP-ribose) (pADPR) could also be detected in spermatid nuclei following gamma-irradiation or H2O2 treatment. Moreover, PARP activation occurs both in spermatocytes and spermatids left to recover after both genotoxic stresses. The NO donors, 3-morpholino-sydnonimine (SIN-1) and S-nitrosoglutathione (SNOG), caused significant SSBs in both spermatocytes and spermatids. The effects of SIN-1 could be prevented by exogenous catalase (CAT), but not superoxide dismutase (SOD), in the cell suspen...Continue Reading

Citations

Oct 19, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jenny Arnold, Tilman Grune
Nov 1, 2007·Cell Biology and Toxicology·A BaumgartnerD Anderson
Apr 25, 2009·DNA and Cell Biology·Martha P Nava-HernándezRicardo M Cerda-Flores
Dec 8, 2009·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalPremendu P Mathur
Feb 4, 2016·Oxidative Medicine and Cellular Longevity·Raquel Frenedoso da SilvaWilma De Grava Kempinas
Sep 8, 2016·International Journal of Molecular Medicine·Peng GaoBing Shen
Sep 29, 2011·Molecular Reproduction and Development·Tushna DehnugaraM R Satyanarayana Rao
Jan 16, 2009·The FEBS Journal·Ankur MaheshwariDeoki Nandan
Sep 7, 2013·International Journal of Molecular Sciences·Paola VillaniEugenia Cordelli
Apr 28, 2004·Molecular Reproduction and Development·Yonka ChristovaRoy Jones
Dec 23, 2009·Free Radical Biology & Medicine·Betul CatalgolTilman Grune

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