Rat growth plate chondrocytes express low levels of 25-hydroxy-1alpha-hydroxylase

The Journal of Steroid Biochemistry and Molecular Biology
Ulrike HügelGünter Klaus

Abstract

Long standing disturbances of Vitamin D-metabolism as well as null-mutant animals for 25-hydroxy-1alpha-hydroxylase results in disorganised growth plates. Cultured chondrocytes were shown to be target for the hydroxylated Vitamin D-metabolites 1alpha,25(OH)(2)D(3) and 24,25(OH)(2)D(3). Because studies on production of these metabolites were inconclusive in in vitro systems, the expression of the Vitamin D-system was examined in rat growth plate chondrocytes in vitro as well as ex vivo. Gene expression for 25-hydroxy-1alpha-hydroxylase, 25-hydroxy-24-hydroxylase as well as Vitamin D-receptor and collagen II and X were analysed on mRNA level by RT-PCR and quantitative real-time PCR, on protein level by western blotting and by immunohistochemistry in isolated growth plate chondrocytes or intact growth plates. Compared to UMR or CaCo(2) cells and renal homogenates cultured growth plate chondrocytes expressed low levels of 25-hydroxy-1alpha-hydroxylase mRNA and 25-hydroxy-24-hydroxylase mRNA. The expression of both was modulated by 25(OH)D(3), but 1alpha,25(OH)(2)D(3) affected only 25-hydroxy-24-hydroxylase. These data were confirmed by Western blotting. Immunohistochemistry demonstrated predominant staining for 25-hydroxy-1alpha-hy...Continue Reading

Citations

Jul 8, 2008·Molecular Aspects of Medicine·Paul H Anderson, Gerald J Atkins
Oct 9, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Jia LiDorothy Teegarden
Apr 22, 2008·Archives of Biochemistry and Biophysics·René St-Arnaud
Apr 30, 2014·Journal of Cellular Physiology·Frances C BachMarianna A Tryfonidou
Sep 23, 2010·American Journal of Physiology. Endocrinology and Metabolism·M A TryfonidouM Karperien

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.