PMID: 6111347Jan 15, 1981Paper

Rat hair follicle and epidermal transglutaminases. Biochemical and immunochemical isoenzymes

Biochimica Et Biophysica Acta
L L Peterson, M M Buxman

Abstract

Epidermal and hair follicle transglutaminases (1,4-alpha-D-glucan: orthophosphate alpha-D-glucosyltransferase EC 2.4.1.1) were differentially isolated and subsequently purified from newborn or 4-5-day-old rats. Both enzymes migrated identically on ion-exchange chromatography but were widely separated by block electrophoresis, with the epidermal enzyme migrating further toward the anode. Each enzyme was finally purified by gel filtration. Epidermal transglutaminase had an apparent molecular weight of 56 000-58 000 in this medium and in gels containing sodium dodecyl sulfate (SDS), while hair follicle transglutaminase had a molecular weight of 52 000-54 000 and was reduced to two apparently identical subunits of a molecular weight of 27 000 by denaturing media. Antiserum specific to each transglutaminase was produced in chickens; when conjugated to fluorescein these antisera localized the enzymes to the granular layer of epidermis and the inner root sheath of follicles, respectively.

References

Jan 1, 1977·The Journal of Investigative Dermatology·H Ogawa, L A Goldsmith
Apr 1, 1979·The Journal of Investigative Dermatology·M M BuxmanK A Holbrook
May 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M M Buxman, K D Wuepper
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S I Chung, J E Folk
Dec 8, 1972·Annals of the New York Academy of Sciences·F Duckert
Sep 7, 1967·Biochemical and Biophysical Research Communications·A L ShapiroJ V Maizel

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Citations

Jan 1, 1984·Molecular and Cellular Biochemistry·L L Peterson, K D Wuepper
Jan 1, 1990·Archives of Dermatological Research·M M OgawaT Nishikawa
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J G ZettergrenK D Wuepper
Apr 1, 1984·The Journal of Clinical Investigation·L L Peterson, K D Wuepper
Dec 1, 1986·The Journal of Dermatology·J NakayamaH Urabe
Jan 1, 1990·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·D BroekaertJ Marquet
Jan 1, 1984·Molecular and Cellular Biochemistry·J A Rothnagel, G E Rogers
Jul 1, 1983·The Journal of Investigative Dermatology·L L PetersonK D Wuepper
Jul 1, 1983·The Journal of Investigative Dermatology·L L Peterson, K D Wuepper
Jan 1, 1986·Differentiation; Research in Biological Diversity·N L ParenteauR H Rice

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