PMID: 1201147Nov 1, 1975Paper

Rat heart lipoprotein lipase

Atherosclerosis
J S TwuM C Schotz

Abstract

Rat heart lipoprotein lipase was highly purified by affinity chromatography using heparin-Sepharose 4B. When extracts of acetone powder were applied to columns, lipase activity was firmly bound to the gel matrix and was later eluted with 1.5 M NaCl. At this stage the eluted enzyme was purified 1500-fold. Disc gel electrophoresis yielded a single protein band corresponding with the enzyme activity. The apparent molecular weight was 60,000. The purified enzyme was highly unstable; however, its activity could be partially stabilized by glycerol or ethylene glycol. In studying the purified enzyme we observed: (i) a cofactor in serum was required for full enzymatic activity; ApoLp-Glu could be substituted for this cofactor; (ii) ApoLp-Ser was inhibitory to lipase activity; (iii) NaCl and protamine sulfate also markedly inhibited the lipase activity; (iv) heparin stimulated the enzymatic activity.

References

Oct 1, 1971·The Biochemical Journal·P H Iverius
Apr 8, 1974·Biochemical and Biophysical Research Communications·P J Dolphin, D Rubinstein
Sep 1, 1973·FEBS Letters·H Greten, B Walter
Nov 1, 1973·Biochemical and Biophysical Research Communications·H JansenW C Hülsmann
Aug 11, 1972·Biochimica Et Biophysica Acta·M C Schotz, A S Garfinkel
Jan 31, 1972·Biochemical and Biophysical Research Communications·W V Brown, M L Baginsky
Dec 28, 1964·Annals of the New York Academy of Sciences·J T CLARKE

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Citations

Apr 26, 1977·Biochimica Et Biophysica Acta·L C HensonI Harary
Nov 24, 1977·Biochimica Et Biophysica Acta·M C SchotzJ Borensztajn
Oct 26, 1979·Biochimica Et Biophysica Acta·L WallinderT Olivecrona
Jan 1, 1991·The International Journal of Biochemistry·M J LaDuW K Palmer
Feb 1, 1985·The Journal of Clinical Investigation·C S WangP Alaupovic
Jan 1, 1983·Progress in Lipid Research·D QuinnR L Jackson
Dec 17, 1975·Biochimica Et Biophysica Acta·L C Henson, M C Schotz
May 11, 1987·Life Sciences·J BrickK DeTurck
Feb 1, 1976·Thrombosis Research·L M Hiebert, L B Jaques
Oct 1, 1987·Analytical Biochemistry·J W GoersM C Schotz
May 15, 1981·Biochemical and Biophysical Research Communications·D L SeversonA Kryski
Sep 15, 1977·European Journal of Biochemistry·G Bengtsson, T Olivecrona
Jan 1, 1981·Journal of Clinical Microbiology·S K MaitraM C Schotz

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