PMID: 9164856May 15, 1997Paper

Rat hepatic glutaminase: identification of the full coding sequence and characterization of a functional promoter

The Biochemical Journal
M I Chung-BokM Watford

Abstract

Glutamine catabolism in mammalian liver is catalysed by a unique isoenzyme of phosphate-activated glutaminase. The full coding and 5' untranslated sequence for rat hepatic glutaminase was isolated by screening lambda ZAP cDNA libraries and a Charon 4a rat genomic library. The sequence produces a mRNA 2225 nt in length, encoding a polypeptide of 535 amino acid residues with a calculated molecular mass of 59.2 kDa. The deduced amino acid sequence of rat liver glutaminase shows 86% similarity to that of rat kidney glutaminase and 65% similarity to a putative glutaminase from Caenorhabditis elegans. A genomic clone to rat liver glutaminase was isolated that contains 3.5 kb of the gene and 7.5 kb of the 5' flanking region. The 1 kb immediately upstream of the hepatic glutaminase gene (from -1022 to +48) showed functional promoter activity in HepG2 hepatoma cells. This promoter region did not respond to treatment with cAMP, but was highly responsive (10-fold stimulation) to the synthetic glucocorticoid dexamethasone. Subsequent 5' deletion analysis indicated that the promoter region between -103 and +48 was sufficient for basal promoter activity. This region does not contain an identifiable TATA element, indicating that transcription...Continue Reading

Citations

May 12, 2009·Neurochemistry International·Vanessa de la RosaJavier Márquez
May 12, 2009·Neurochemistry International·Javier MárquezJosé A Campos-Sandoval
May 12, 2009·Neurochemistry International·Monika Szeliga, Marta Obara-Michlewska
Feb 3, 2007·The International Journal of Biochemistry & Cell Biology·José A Campos-SandovalJavier Márquez
Dec 25, 2016·Neurochemical Research·Javier MárquezFernando Rodríguez de Fonseca
Jan 24, 2017·Future Medicinal Chemistry·William P KattRichard A Cerione
May 4, 1999·The International Journal of Biochemistry & Cell Biology·R McCauleyJ C Hall
Mar 1, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Maha El HageGuy Martin
Apr 8, 2006·Physiological Reviews·Béatrice DesvergneWalter Wahli
May 1, 2010·Biomolecular Concepts·Javier MárquezFrancisco J Alonso
Mar 29, 2000·The Journal of Nutrition·M Watford
Jun 5, 2002·Physiological Genomics·L David PorterNorman P Curthoys
Apr 20, 2004·The Biochemical Journal·David Baglietto-VargasJ Carlos Aledo
Nov 20, 2019·International Journal of Biological Macromolecules·Tahany M MaharemYoussef A Said
Mar 23, 2021·Biochimie·Igor M FerreiraAndre Lb Ambrosio
Mar 27, 2001·Biochimica Et Biophysica Acta·L TaylorN P Curthoys
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G WuM Watford

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