PMID: 2123510Jan 1, 1990Paper

Rat hypothalamic corticotropin-releasing hormone secretion in vitro is stimulated by interleukin-1 in an eicosanoid-dependent manner

Life Sciences
R BernardiniG P Chrousos

Abstract

We studied the effect of interleukin-1 alpha (IL-1) on corticotropin-releasing hormone (CRH) secretion by explanted rat hypothalami in vitro. We also assessed possible mediation of arachidonic acid metabolites on IL-1-stimulated CRH secretion, by preincubating hypothalami with the cyclooxygenase inhibitor indomethacin (INDO, 1 microM), the lipoxygenase and cyclooxygenase inhibitor eicosatetraynoic acid (ETYA, 10 microM), or the lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA, up to 30 microM). In additional experiments, prostaglandins (PG) E2 and F2 alpha were added to the cultures treated with INDO or ETYA. Finally, we investigated the effect of dexamethasone (DEX) on IL-1-stimulated CRH secretion. IL-1 stimulated immunoreactive CRH (iCRH) secretion by explanted hypothalami in a concentration-dependent fashion. Both INDO and ETYA inhibited IL-1-(10nM)-stimulated iCRH secretion, whereas NDGA did not have any effect. The addition of PGF2 alpha (10 nM) restored the secretion of iCRH inhibited by INDO. DEX treatment significantly inhibited IL-1-stimulated iCRH release. Our results suggest that the stimulatory effect of IL-1 on the hypothalamic CRH neuron is mediated by the cyclooxygenase metabolites of arachidonic acid, and...Continue Reading

References

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Citations

Feb 1, 1995·Molecular Neurobiology·F ShintaniM Asai
Mar 1, 1993·Pharmacology, Biochemistry, and Behavior·M P IuratoR Bernardini
May 1, 1996·Pharmacology, Biochemistry, and Behavior·J C BuckinghamJ G Philip
Apr 29, 1991·Biochimica Et Biophysica Acta·M L PatchettH W Morgan
Dec 1, 1991·Molecular and Cellular Endocrinology·N J Busbridge, A B Grossman
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Dec 22, 1998·Biochimica Et Biophysica Acta·G L MendzR L Ferrero
Sep 6, 2002·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A KellesJ Tack
Jan 23, 1999·International Reviews of Immunology·R C Gaillard
Sep 1, 1994·Trends in Endocrinology and Metabolism : TEM·R C Gaillard
Oct 23, 1997·The Journal of Comparative Neurology·S Lacroix, S Rivest
Jun 10, 2003·Chembiochem : a European Journal of Chemical Biology·Natalya Lozovaya, Andrew D Miller
Jan 1, 1990·Fundamental & Clinical Pharmacology·P J Neveu, M Le Moal
Dec 1, 1991·American Journal of Reproductive Immunology : AJRI·N AzadN V Emanuele
Mar 26, 1999·Scandinavian Journal of Rheumatology·H DemirY Ozügül
Aug 16, 2019·Frontiers in Neuroendocrinology·L S KalinichenkoC P Müller
Aug 2, 2014·Behavioural Pharmacology·Joseph M CatanzaroTerrence Deak
May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Inssaf BerkiksA El Hessni

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