Rat liver preservation by hypothermic oscillating liver perfusion compared to simple cold storage

Cryobiology
P DutkowskiT Junginger

Abstract

Rat livers were preserved hypothermically for 10 or 24 h in vitro as if for transplantation. Two methods of preservation were compared using physiological and biochemical parameters: simple storage and oscillating perfusion. By measuring the nucleotides after preservation the calculated energy charge was significantly higher after 10 and 24 h of oscillating perfusion compared to the simple storage group. In addition, a significant energy charge loading was demonstrated by 10 h oscillating perfusion compared to the initial value prior to perfusion. The oscillating, computer-controlled perfusion permits continuous monitoring of perfusate temperature, O2 consumption, pCO2, portal vein pressure, and pH and also automatic sample collection and pH compensation. In addition, the perfusate can be easily exchanged by using two different pumps or be rewarmed by a heat exchanger. For measuring of short-lived metabolites (interleukins, oxygen radicals, prostaglandins) sampling can be performed directly out of the vena cava outflow. pH and temperature stability was maintained by a data acquisition and controlling system. Because of a special designed liver chamber a combination of storage and perfusion with or without substrates was possibl...Continue Reading

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Citations

Oct 27, 2006·Annals of Biomedical Engineering·Arjan van der PlaatsG Rakhorst
May 12, 2001·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·M Kukan, P S Haddad
Feb 4, 2010·Current Opinion in Organ Transplantation·Diethard Monbaliu, John Brassil
Feb 4, 2010·Current Opinion in Organ Transplantation·Olivier de RougemontPierre-Alain Clavien
Feb 18, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·T MinorF Dombrowski
Sep 4, 2015·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·P Dutkowski, Philipp Dutkowski
Oct 21, 2005·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Nils A 't HartRutger J Ploeg
Apr 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P DutkowskiP A Clavien
May 12, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·T MinorF Dombrowski
Dec 4, 2013·Journal of Hepatology·P DutkowskiPierre-Alain Clavien
May 24, 2014·Transplant International : Official Journal of the European Society for Organ Transplantation·Andrea Schlegel, P Dutkowski
Jun 23, 2015·Current Transplantation Reports·Andrea SchlegelP Dutkowski
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May 1, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S A KarangwaR J Porte
Feb 8, 2018·Oxidative Medicine and Cellular Longevity·Anne C Van ErpJean-Paul Decuypere
Sep 19, 2017·Current Opinion in Organ Transplantation·Andrea SchlegelPhilipp Dutkowski
Mar 23, 2018·Current Transplantation Reports·Andrea SchlegelPhilipp Dutkowski
Jun 6, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Weiyang HeQifa Ye
Dec 27, 2017·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·R Cutler Quillin, James V Guarrera

Related Concepts

Adenine Nucleotides
Clinical Engineering
Diastolic Blood Pressure
Cold Temperature
Glycolysis
Hydrogen-Ion Concentration
Lactate Dehydrogenase
Liver
Liver Glycogen
Organ Preservation

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