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Rat liver thioredoxin and thioredoxin reductase: purification and characterization

Biochemistry

Dec 21, 1982

M Luthman, A Jay Holmgren

PMID: 7159551

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Abstract

A reproducible scheme has been developed for the preparation of rat liver thioredoxin and thioredoxin reductase (EC 1.6.4.5) by using assays based on reduction of insulin and 5,5'-dithiobis(2-nitrobenzoic acid), respectively. Both proteins were purified to homogeneity, as judged from po...read more

Mentioned in this Paper

Thioredoxin (human)
Cattle calf (organism)
Ion-Exchange Chromatography Procedure
NADP
Metabolic Inhibition
Bacterial Proteins
Arsenite
Bos taurus
Oxidoreductase
Aggregation
Paper Details
References
  • References11
  • Citations292
12345...

Rat liver thioredoxin and thioredoxin reductase: purification and characterization

Biochemistry

Dec 21, 1982

M Luthman, A Jay Holmgren

PMID: 7159551

DOI:

Abstract

A reproducible scheme has been developed for the preparation of rat liver thioredoxin and thioredoxin reductase (EC 1.6.4.5) by using assays based on reduction of insulin and 5,5'-dithiobis(2-nitrobenzoic acid), respectively. Both proteins were purified to homogeneity, as judged from po...read more

Mentioned in this Paper

Thioredoxin (human)
Cattle calf (organism)
Ion-Exchange Chromatography Procedure
NADP
Metabolic Inhibition

Related Papers

Paper Details
References
  • References11
  • Citations292
12345...

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