PMID: 9539110Apr 16, 1998Paper

Rat mitochondrial glycerol-3-phosphate dehydrogenase gene: multiple promoters, high levels in brown adipose tissue, and tissue-specific regulation by thyroid hormone

DNA and Cell Biology
D W GongMarc L Reitman

Abstract

Mitochondrial FAD-linked glycerol-3-phosphate dehydrogenase (mtGPDH) is one of the two enzymes of the glycerol phosphate shuttle. This shuttle transfers reducing equivalents from the cytoplasm to the mitochondria in a unidirectional, exothermic manner. Here, the isolation and characterization of the rat nuclear gene (Gpd2) encoding mtGPDH is reported. The mtGPDH gene spans 100 kb and consists of 17 exons. The use of alternate promoters was suggested by the presence of three different first exons and confirmed by transient expression for two of them. The first exons are expressed in a tissue-restricted manner. Exon 1a was found primarily in brain, exon 1b was used in all tissues examined, and exon 1c was detected predominantly in testis. Depending on the tissue, different transcript lengths were also observed: 5.9 kb (all tissues), 3.6 kb (skeletal muscle), and 2.5 kb (testis). The length isoforms are attributable to alternate splicing and polyadenylation site use. Very high mtGPDH mRNA levels were found in brown adipose tissue, 75 fold greater than in white adipose tissue. Thyroid hormone increased mtGPDH mRNA levels in liver and heart but not in brown adipose tissue, brain, or testis. This pattern corresponds to that of thyroi...Continue Reading

References

Jun 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D RicquierF Bouillaud
Jan 1, 1987·Annual Review of Genetics·U Schibler, F Sierra
Jun 1, 1995·Current Opinion in Cell Biology·C J Decker, R Parker
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·S Müller, H J Seitz
Jan 1, 1995·Annual Review of Nutrition·H C Freake, J H Oppenheimer
Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Dec 1, 1995·Thyroid : Official Journal of the American Thyroid Association·J E Silva
Dec 1, 1996·Archives of Biochemistry and Biophysics·R A KozaL P Kozak
Dec 1, 1964·The American Journal of Physiology·R R CHAFFEER E SMITH

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Citations

Nov 18, 2008·Human Genetics·Hussein DaoudSylvain Briault
Jul 25, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·U BhattacharyyaA K Medda
Jul 25, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·W BerradaN Bourhim
May 10, 2008·Thyroid : Official Journal of the American Thyroid Association·Yves DebaveyeGreet Van den Berghe
Feb 19, 2008·Thyroid : Official Journal of the American Thyroid Association·Miriam O Ribeiro
Feb 24, 2007·The Journal of Clinical Investigation·Marija TrajkovicHeike Heuer
Nov 12, 2005·Bioscience Reports·J Enrique Silva
Dec 12, 2012·Biochimica Et Biophysica Acta·Tomáš MráčekJosef Houštěk
Sep 24, 1999·Biochemical and Biophysical Research Communications·M GudayolR Gomis
Dec 3, 2013·Cell Reports·Irina G ShabalinaJan Nedergaard
May 27, 2003·Biochimica Et Biophysica Acta·Bernhard Kadenbach
Aug 7, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Dave Speijer
Oct 27, 2018·Molecular Biology and Evolution·Adam G CiezarekVincent Savolainen
Apr 8, 2006·Physiological Reviews·J Enrique Silva
Oct 21, 2017·Nature Reviews. Endocrinology·Matthias J Betz, Sven Enerbäck
Aug 10, 2002·American Journal of Physiology. Endocrinology and Metabolism·Angel A ZaninovichKarl Hagmüller
Jul 2, 2003·Journal of Applied Physiology·Angel A ZaninovichKarl Hagmuller
Aug 4, 2018·Frontiers in Endocrinology·Ruy A Louzada, Denise P Carvalho
Aug 24, 2000·The Journal of Biological Chemistry·Q GongM J MacDonald
Sep 9, 2020·Endocrine Reviews·Traci E LaMoia, Gerald I Shulman

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