Feb 11, 2014

Rat model of veno-arterial extracorporeal membrane oxygenation

Journal of Translational Medicine
Ayyaz AliZiad Ali

Abstract

We aim to develop a rat model of veno-arterial extracorporeal membrane oxygenation (VA-ECMO). VA-ECMO was established in twelve Male Sprague-Dawley rats (250-350 g) through cannulation of the right jugular vein for venous drainage and the right femoral artery for arterial reinfusion. Arterial blood pressure was measured using a conductance catheter through cannulation of the left carotid artery. Heart rate was monitored by electrocardiography and arterial blood gas parameters with a blood gas analyzer. The VA-ECMO circuit was tested by subjecting the rats to hypoxic cardiac arrest with resuscitation using VA-ECMO. Both load-dependent and load-independent measures of myocardial contractility were measured using pressure-volume loop analysis to confirm restoration of myocardial function post-resuscitation. Following hypoxic cardiac arrest VA-ECMO provided sufficient oxygenation to support the circulation. The haemodynamic and blood gas parameters were maintained at transition and during ECMO. All animals were resuscitated, regained cardiac function and were able to be weaned off ECMO post-resuscitation. We have established a safe, high-throughput, economical, functioning rat model of VA-ECMO.

Mentioned in this Paper

Metabolic Process, Cellular
Ischemia
Myocardial Contraction
Alveolar Ventilation Function
Normal saline
Region of Neck (Body Structure)
Body Parts - Cannula
Specimen Type - Cannula
Lung
Calcium

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Cardiogenic Shock

Cardiogenic shock is a devastating consequence of acute myocardial infarction and is associated with an extremely high mortality. Here is the latest research.

Related Papers

Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions
Oliver HusserMichael Hilker
Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies
Talya K E FreyK Sarah Hoehn
© 2020 Meta ULC. All rights reserved