Rat Mrp2 gene expression is regulated by an interleukin-1β-stimulated biphasic response with enhanced transcription and subcellular shuttling of YB-1

European Journal of Cell Biology
Peter R MertensAndreas Geier

Abstract

Expression of hepatobiliary transporters is decreased during endotoxemia. Reduction of Mrp2 is mediated by IL-1β-dependent signals but underlying mechanisms are still unclear. YB-1 is a predominantly cytoplasmic protein that translocates to the nucleus in response to various stimuli. Previously we have shown that YB-1 down-regulates Mrp2 expression in vitro. Therefore we investigated the potential role of YB-1 as regulator of hepatic acute phase genes. Liver sections from LPS-injected rats (20 h) were stained with YB-1-specific antibodies. Real-time RT-PCR quantification was performed for Mrp2, MMP-2 and YB-1. YB-1 protein was quantified from IL-1β- or TNFα-stimulated rat hepatoma cells (FaO) and the localization of a YFP-YB-1-CFP fusion protein was visualized by confocal microscopy in HepG2 human hepatocellular carcinoma cells. ChIP-assays and EMSA were performed to analyze YB-1 binding to DNA promoter elements. In endotoxemic livers Mrp2 mRNA was down-regulated by 80%, while YB-1 mRNA expression increased 2.5-fold. Immunohistochemical staining showed a marked up-regulation and predominant nuclear localization of YB-1 protein in LPS challenged rats. In FAO cells IL-1β incubation increased cytoplasmic YB-1 protein content up to...Continue Reading

References

Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D K DidierB D Schwartz
May 1, 1987·Journal of Bacteriology·P G JonesF C Neidhardt
Apr 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A P Wolffe
Oct 22, 1998·The New England Journal of Medicine·M TraunerJ L Boyer
Oct 12, 2000·Cellular and Molecular Life Sciences : CMLS·P Cartwright, K Helin
Nov 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E E CapowskiJ S Malter
Feb 27, 2003·The Journal of Pharmacology and Experimental Therapeutics·Jukka HakkolaMagnus Ingelman-Sundberg
Mar 29, 2003·Physiological Reviews·Michael Trauner, James L Boyer
Apr 16, 2003·The Journal of Biological Chemistry·Karsten JurchottHans-Dieter Royer
May 14, 2003·Oncogene·You Fang ZhangAntony W Braithwaite
Jun 20, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kimitoshi KohnoMichihiko Kuwano
Jul 29, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Andreas GeierCarsten Gartung
Sep 10, 2003·Biochemical and Biophysical Research Communications·A GeierC Gartung
Oct 21, 2004·Nucleic Acids Research·Maxim A SkabkinLev P Ovchinnikov
Dec 24, 2004·The Journal of Biological Chemistry·Abdelaziz En-NiaPeter R Mertens
Jul 9, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gernot ZollnerMichael Trauner
Aug 12, 2005·Journal of the American Society of Nephrology : JASN·Claudia R C van RoeyenPeter R Mertens
Sep 15, 2005·Oncogene·Craig HomerAntony W Braithwaite
Dec 16, 2005·Molecular and Cellular Biology·Valentina EvdokimovaPoul H B Sorensen

❮ Previous
Next ❯

Citations

Feb 25, 2014·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jonathan A LindquistPeter R Mertens
Jul 23, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lydia HanssenUte Raffetseder
Sep 28, 2018·Cell Communication and Signaling : CCS·Jonathan A Lindquist, Peter R Mertens

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.